Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI
Abstract
The authors have cloned segments of yeast DNA containing the centromere XI-linked MET14 gene. This was done by selecting directly in Saccharomyces cerevisiae for complementation of a met14 mutation after transformation with a hybrid plasmid DNA genomic library. Generic evidence indicates that functional centromere DNA (CEN11) from chromosome XI is also contained on the segment of S. cerevisiae DNA cloned in pYe(MET14)2. This plasmid is maintained stably in budding S. cerevisiae cultures and segregates predominantly 2 + :2- through meiosis. The CEN11 element has been subcloned in vector YRp7' on an S. cerevisiae DNA fragment 900 base pairs in length (pYe(CEN11)10). The mitotic and meiotic behavior of plasmids containing CEN11 plus a DNA replicator (ars) indicates that the centromere DNA sequences enable these plasmids to function as true minichromosomes in S. cerevisiae.
- Authors:
- Publication Date:
- Research Org.:
- Dept. of Biological Sciences, Univ. of California, Santa Barbara, CA 93106
- OSTI Identifier:
- 5652999
- Resource Type:
- Journal Article
- Journal Name:
- Mol. Cell. Biol.; (United States)
- Additional Journal Information:
- Journal Volume: 2:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; PLASMIDS; PHYSIOLOGY; STRUCTURE-ACTIVITY RELATIONSHIPS; SACCHAROMYCES CEREVISIAE; CELL CULTURES; CHROMOSOMAL ABERRATIONS; GENOME MUTATIONS; CHROMOSOMES; DNA REPLICATION; DNA-CLONING; HYBRIDOMAS; MEIOSIS; MITOSIS; ONCOGENIC TRANSFORMATIONS; REDUCTIVE EXTRACTION; REPLICONS; ANIMAL CELLS; CELL CONSTITUENTS; CELL DIVISION; CELL TRANSFORMATIONS; CLONING; EXTRACTION; FUNGI; GENES; MICROORGANISMS; MUTATIONS; NUCLEIC ACID REPLICATION; PLANTS; SACCHAROMYCES; SEPARATION PROCESSES; YEASTS; 550400* - Genetics
Citation Formats
Fitzgerald-Hayes, M, Buhler, J M, Cooper, T G, and Carbon, J. Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI. United States: N. p., 1982.
Web. doi:10.1128/MCB.2.1.82.
Fitzgerald-Hayes, M, Buhler, J M, Cooper, T G, & Carbon, J. Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI. United States. https://doi.org/10.1128/MCB.2.1.82
Fitzgerald-Hayes, M, Buhler, J M, Cooper, T G, and Carbon, J. 1982.
"Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI". United States. https://doi.org/10.1128/MCB.2.1.82.
@article{osti_5652999,
title = {Isolation and subcloning analysis of functional centromere DNA(CEN11) from Saccharomyces cerevisiae chromosome XI},
author = {Fitzgerald-Hayes, M and Buhler, J M and Cooper, T G and Carbon, J},
abstractNote = {The authors have cloned segments of yeast DNA containing the centromere XI-linked MET14 gene. This was done by selecting directly in Saccharomyces cerevisiae for complementation of a met14 mutation after transformation with a hybrid plasmid DNA genomic library. Generic evidence indicates that functional centromere DNA (CEN11) from chromosome XI is also contained on the segment of S. cerevisiae DNA cloned in pYe(MET14)2. This plasmid is maintained stably in budding S. cerevisiae cultures and segregates predominantly 2 + :2- through meiosis. The CEN11 element has been subcloned in vector YRp7' on an S. cerevisiae DNA fragment 900 base pairs in length (pYe(CEN11)10). The mitotic and meiotic behavior of plasmids containing CEN11 plus a DNA replicator (ars) indicates that the centromere DNA sequences enable these plasmids to function as true minichromosomes in S. cerevisiae.},
doi = {10.1128/MCB.2.1.82},
url = {https://www.osti.gov/biblio/5652999},
journal = {Mol. Cell. Biol.; (United States)},
number = ,
volume = 2:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}