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Title: Pyropheophorbide und a as a catabolite of ethylene-induced chlorophyll und a degradation

Abstract

An enzyme extract prepared from ethylene-induced degreening Citrus fruits contains chlorophyll (Chl) degrading enzymes. The fate of Chl carbons during an enzymatic degradation was investigated using Chl {und a}-{sup 14}C. Accompanying the disappearance of labelled Chl {und a}, pheophorbide {und a} and pyropheophorbide {und a} appeared and accumulation of pyropheophorbide {und a} was observed. HydroxyChl {und a} was also detected, but this is thought to be an artifact during chromatography. Unlike ethylene-induced Citrus fruits (in vivo), further degradation of pyropheophorbide {und a} did not occur in vitro enzyme system. This suggests that there is a lack of enzyme(s) and/or cofactor(s) for further degradation. It is concluded that Chl {und a} degraded enzymatically by the following order: Chl {und a}, chlorophyllide {und a}, pheophorbide {und a} and pyropheophorbide {und a}.

Authors:
;  [1];  [2]
  1. (Miyazaki Univ. (Japan))
  2. (Miyazaki Medical College (Japan))
Publication Date:
OSTI Identifier:
5644953
Report Number(s):
CONF-9007196-
Journal ID: ISSN 0079-2241; CODEN: PPYSA; TRN: 91-016561
Resource Type:
Conference
Journal Name:
Plant Physiology, Supplement; (USA)
Additional Journal Information:
Journal Volume: 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 14 SOLAR ENERGY; BIOLOGICAL PATHWAYS; CHLOROPHYLL; CATABOLISM; CARBON 14 COMPOUNDS; CHROMATOGRAPHY; CITRUS; ENZYMES; ETHYLENE; TRACER TECHNIQUES; ALKENES; CARBON COMPOUNDS; CARBOXYLIC ACIDS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROCARBONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAGNOLIOPHYTA; MAGNOLIOPSIDA; METABOLISM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PHYTOCHROMES; PIGMENTS; PLANTS; PORPHYRINS; PROTEINS; SEPARATION PROCESSES; 550201* - Biochemistry- Tracer Techniques; 550501 - Metabolism- Tracer Techniques; 140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)

Citation Formats

Shimokawa, Keishi, Hashizume, Akihito, and Shioi, Yuzo. Pyropheophorbide und a as a catabolite of ethylene-induced chlorophyll und a degradation. United States: N. p., 1990. Web.
Shimokawa, Keishi, Hashizume, Akihito, & Shioi, Yuzo. Pyropheophorbide und a as a catabolite of ethylene-induced chlorophyll und a degradation. United States.
Shimokawa, Keishi, Hashizume, Akihito, and Shioi, Yuzo. Tue . "Pyropheophorbide und a as a catabolite of ethylene-induced chlorophyll und a degradation". United States.
@article{osti_5644953,
title = {Pyropheophorbide und a as a catabolite of ethylene-induced chlorophyll und a degradation},
author = {Shimokawa, Keishi and Hashizume, Akihito and Shioi, Yuzo},
abstractNote = {An enzyme extract prepared from ethylene-induced degreening Citrus fruits contains chlorophyll (Chl) degrading enzymes. The fate of Chl carbons during an enzymatic degradation was investigated using Chl {und a}-{sup 14}C. Accompanying the disappearance of labelled Chl {und a}, pheophorbide {und a} and pyropheophorbide {und a} appeared and accumulation of pyropheophorbide {und a} was observed. HydroxyChl {und a} was also detected, but this is thought to be an artifact during chromatography. Unlike ethylene-induced Citrus fruits (in vivo), further degradation of pyropheophorbide {und a} did not occur in vitro enzyme system. This suggests that there is a lack of enzyme(s) and/or cofactor(s) for further degradation. It is concluded that Chl {und a} degraded enzymatically by the following order: Chl {und a}, chlorophyllide {und a}, pheophorbide {und a} and pyropheophorbide {und a}.},
doi = {},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 93:1,
place = {United States},
year = {1990},
month = {5}
}

Conference:
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