Preparation of sup 2 H- and sup 3 H-labeled phaseic acid and dihydrophaseic acid as standards for determination of abscisic acid metabolites in tomato fruit

Conference · Tue May 01 00:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5643851

Kubik, M; Buta, J G ^[1]

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1. USDA, Beltsville, MD (USA)

There have been reports that the level of abscisic acid (ABA) increases during the cold storage of tomatoes. However, the important ABA metabolites, phaseic acid (PA) and dihydrophaseic acid (DPA) were never quantitatively determined in such a system. In order to obtain the labeled standards for quantitative determination of those compounds by GC-MS-SIM, we fed bean plants with 6,6,6-({sup 2}H{sub 3})-ABA (mean isotopic enrichment 60%) with addition of about 10{sup 5} Bq per mg of ({sup 3}H)-ABA. After 100 hours the plants were harvested and extracted with acetone. The extract were purified by solvent partitioning and, Prep-Sep amino column and on an HPLC C{sub 18} reverse phase column. Two major radioactive metabolites of ABA were obtained and identified by GC-MS as PA and DPA. Some results on the quantitation of ABA, PA and DPA in tomato fruit after cold storage will be presented.

• OSTI ID: 5643851
• Report Number(s): CONF-9007196-; CODEN: PPYSA
• Journal Information: Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ABSCISIC ACID
METABOLITES
DEUTERIUM COMPOUNDS
TOMATOES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AUXINS
CARBOXYLIC ACIDS
FOOD
FRUITS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANT GROWTH REGULATORS
550501* - Metabolism- Tracer Techniques
553000 - Agriculture & Food Technology