Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro

Journal Article · Mon Aug 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.15.5698· OSTI ID:5642026

Kawasaki, E S; Clark, S S; Coyne, M Y; Smith, S D; Champlin, R; Witte, O N; McCormick, F P ^[1]

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1. Cetus Corp., Emeryville, CA (USA)

The Philadelphia chromosome is present in more than 95% of chronic myeloid leukemia patients and 13% of acute lymphocytic leukemia patients. The Philadelphia translocation, t(9;22), fuses the BCR and ABL genes resulting in the expression of leukemia-specific, chimeric BCR-ABL messenger RNAs. To facilitate diagnosis of these leukemias, the authors have developed a method of amplifying and detecting only the unique mRNA sequences, using an extension of the polymerase chain reaction technique. Diagnosis of chronic myeloid and acute lymphocytic leukemias by this procedure is rapid, much more sensitive than existing protocols, and independent of the presence or absence of an identifiable Philadelphia chromosome.

• OSTI ID: 5642026
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:15; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LEUKEMIA
DIAGNOSIS
MESSENGER-RNA
MOLECULAR STRUCTURE
BIOLOGICAL MARKERS
CHROMOSOMAL ABERRATIONS
DNA SEQUENCING
KARYOTYPE
PATIENTS
PHILADELPHIA CHROMOSOME
CHROMOSOMES
DISEASES
HEMIC DISEASES
IMMUNE SYSTEM DISEASES
MUTATIONS
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques