skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: A kinetic and microautoradiographic study of sup 14 C-sucrose translocation into developing wheat grains

Abstract

The kinetics of {sup 14}C-photosynthate import by developing wheat grains was followed after pulse-labeling the flag leaf with {sup 14}CO{sub 2}. Samples were collected from four successive points along the transport pathway to and within the grain: exuding aphid stylets on the peduncle, exuding grain pedicels, the grain crease tissues, and the liquid contents of the endosperm cavity. In addition, microautoradiographs were prepared of the grain crease tissues during movement of the {sup 14}C pulse into the grain. At all times, sucrose accounted for 93 to 97% of the total {sup 14}C present at all four sampling sites. The main features of the {sup 14}C kinetics could be accounted for by a simple compartmental model consisting of sucrose pools in series. Microautoradiographs of the crease tissues showed fairly uniform labeling of vascular parenchyma at all times, with a sharp gradient in labeling across the chalaza to the nucellus. Thus the principal resistance to post-phloem solute transport through the maternal tissues appears to be in the symplastic pathway across the chalaza.

Authors:
;  [1]
  1. Washington State Univ., Pullman (United States)
Publication Date:
OSTI Identifier:
5640341
Report Number(s):
CONF-9107184-
Journal ID: ISSN 0079-2241; CODEN: PPYSA
Resource Type:
Conference
Journal Name:
Plant Physiology, Supplement; (United States)
Additional Journal Information:
Journal Volume: 96:1; Conference: Annual meeting of the American Society of Plant Physiology, Albuquerque, NM (United States), 28 Jul - 1 Aug 1991; Journal ID: ISSN 0079-2241
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; SACCHAROSE; METABOLISM; WHEAT; PHYSIOLOGY; AUTORADIOGRAPHY; BIOLOGICAL PATHWAYS; CARBON 14 COMPOUNDS; CARBON DIOXIDE; LEAVES; PHOTOSYNTHESIS; TRANSLOCATION; CARBOHYDRATES; CARBON COMPOUNDS; CARBON OXIDES; CEREALS; CHALCOGENIDES; CHEMICAL REACTIONS; DISACCHARIDES; GRAMINEAE; LABELLED COMPOUNDS; LILIOPSIDA; MAGNOLIOPHYTA; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; SACCHARIDES; SYNTHESIS; 550501* - Metabolism- Tracer Techniques

Citation Formats

Wang, Ning, and Fisher, D B. A kinetic and microautoradiographic study of sup 14 C-sucrose translocation into developing wheat grains. United States: N. p., 1991. Web.
Wang, Ning, & Fisher, D B. A kinetic and microautoradiographic study of sup 14 C-sucrose translocation into developing wheat grains. United States.
Wang, Ning, and Fisher, D B. 1991. "A kinetic and microautoradiographic study of sup 14 C-sucrose translocation into developing wheat grains". United States.
@article{osti_5640341,
title = {A kinetic and microautoradiographic study of sup 14 C-sucrose translocation into developing wheat grains},
author = {Wang, Ning and Fisher, D B},
abstractNote = {The kinetics of {sup 14}C-photosynthate import by developing wheat grains was followed after pulse-labeling the flag leaf with {sup 14}CO{sub 2}. Samples were collected from four successive points along the transport pathway to and within the grain: exuding aphid stylets on the peduncle, exuding grain pedicels, the grain crease tissues, and the liquid contents of the endosperm cavity. In addition, microautoradiographs were prepared of the grain crease tissues during movement of the {sup 14}C pulse into the grain. At all times, sucrose accounted for 93 to 97% of the total {sup 14}C present at all four sampling sites. The main features of the {sup 14}C kinetics could be accounted for by a simple compartmental model consisting of sucrose pools in series. Microautoradiographs of the crease tissues showed fairly uniform labeling of vascular parenchyma at all times, with a sharp gradient in labeling across the chalaza to the nucellus. Thus the principal resistance to post-phloem solute transport through the maternal tissues appears to be in the symplastic pathway across the chalaza.},
doi = {},
url = {https://www.osti.gov/biblio/5640341}, journal = {Plant Physiology, Supplement; (United States)},
issn = {0079-2241},
number = ,
volume = 96:1,
place = {United States},
year = {Wed May 01 00:00:00 EDT 1991},
month = {Wed May 01 00:00:00 EDT 1991}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: