Physiological profiling of indigenous aquatic microbial communities to determine toxic effects of metals
- Idaho National Engineering and Environmental Lab., Idaho Falls, ID (United States)
- Dynamac, Kennedy Space Center, FL (United States)
Conventional bioassays for environmental assessment frequently rely on nonindigenous single species. The authors employed an assay in which whole environmental samples were distinguished by the ability of the native heterotrophic microbial communities to oxidize 95 different sole carbon sources generating a community-level physiological profile (CLPP). The average metabolic response (AMR) to the 95 variables defining the CLPP was used in laboratory bioassay studies with copper to construct dose-response curves over several different periods of exposure: 1 h (acute), 1 d, 2 d, and 4 d. The acute dose-response of Snake River bacterioplankton communities measured by AMR was compared to the dose-response of Photobacterium phosphoreum (used in the Microtox test) and a proprietary mixed consortia (used in the Polytox test). In laboratory bioassay studies, CLPP, AMR exhibited acute dose-response behavior over a greater range in copper concentrations and with less variability (per dose) than Microtox and Polytox. The acute sensitivity of CLPP AMR to copper was roughly equal to Microtox and much greater than Polytox. After a longer exposure (1 d) to copper, Snake River communities became more sensitive to copper but no additional effect was observed when the exposure was increases to 2 and 4 d. Snake River communities pre-exposed to copper (1 mg/L) for 4 d prior to acute dose-response experiments showed no difference in AMR with respect to doses up to 10 mg/L, indicating the ability of the assay to detect adaptation. Several metal-contaminated streams in Idaho were used to field validate the CLPP approach for detecting impacts of metals in the environment. The response profiles of the bacterioplankton from two downstream sites receiving metal laden mine drainage were compared to those from reference sites upstream and further downstream of the location receiving the mine drainage.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC07-76ID01570; AC07-94ID13223
- OSTI ID:
- 563941
- Journal Information:
- Environmental Toxicology and Chemistry, Vol. 16, Issue 11; Other Information: PBD: Nov 1997
- Country of Publication:
- United States
- Language:
- English
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