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Title: Phenylpropanoid metabolism in suspension cultures of Vanilla planifolia Andr

Abstract

Feeding of cinnamic acid and ferulic acid to non-treated and chitosan-treated cell suspension cultures of Vanilla planifolia resulted in the formation of trace amounts of p-hydroxybenzoic acid (5.2 micrograms per gram fresh weight of cells) and vanillic acid (6.4 micrograms per gram fresh weight of cells), respectively. Addition of a 4-hydroxycinnamate: CoA-ligase inhibitor, 3,4-(methylenedioxy)-cinnamic acid (MDCA), resulted in a reduced biosynthesis of ligneous material with a simultaneous significant increased vanillic acid formation (around 75 micrograms per gram fresh weight of cells). A K{sub i} of 100 micromolar for 4-hydroxycinnamate: CoA-ligase in a crude preparation was estimated for this inhibitor. It is suggested that the conversion of cinnamic acids into benzoic acids does not involve cinnamoyl CoA esters as intermediates. Feeding of {sup 14}C-cinnamic acid and {sup 14}C-ferulic acid to cells treated with MDCA indicate that cinnamic acid, but not ferulic acid, is a precursor of vanillic acid in these cultivated cells of V. planifolia.

Authors:
;  [1]
  1. (ETH Hoenggerberg, Zurich (Switzerland))
Publication Date:
OSTI Identifier:
5628026
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 94:1; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; PROPIONIC ACID; METABOLISM; AROMATICS; BIOLOGICAL PATHWAYS; CARBON 14 COMPOUNDS; CELL CULTURES; CINNAMIC ACID; ENZYME INHIBITORS; LIGASES; LILIOPSIDA; PRECURSOR; TRACER TECHNIQUES; CARBOXYLIC ACIDS; ENZYMES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAGNOLIOPHYTA; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PLANTS; 550201* - Biochemistry- Tracer Techniques; 550501 - Metabolism- Tracer Techniques; 090800 - Biomass Fuels- Production- (1990-)

Citation Formats

Funk, C., and Brodelius, P.E. Phenylpropanoid metabolism in suspension cultures of Vanilla planifolia Andr. United States: N. p., 1990. Web. doi:10.1104/pp.94.1.95.
Funk, C., & Brodelius, P.E. Phenylpropanoid metabolism in suspension cultures of Vanilla planifolia Andr. United States. doi:10.1104/pp.94.1.95.
Funk, C., and Brodelius, P.E. Sat . "Phenylpropanoid metabolism in suspension cultures of Vanilla planifolia Andr". United States. doi:10.1104/pp.94.1.95.
@article{osti_5628026,
title = {Phenylpropanoid metabolism in suspension cultures of Vanilla planifolia Andr},
author = {Funk, C. and Brodelius, P.E.},
abstractNote = {Feeding of cinnamic acid and ferulic acid to non-treated and chitosan-treated cell suspension cultures of Vanilla planifolia resulted in the formation of trace amounts of p-hydroxybenzoic acid (5.2 micrograms per gram fresh weight of cells) and vanillic acid (6.4 micrograms per gram fresh weight of cells), respectively. Addition of a 4-hydroxycinnamate: CoA-ligase inhibitor, 3,4-(methylenedioxy)-cinnamic acid (MDCA), resulted in a reduced biosynthesis of ligneous material with a simultaneous significant increased vanillic acid formation (around 75 micrograms per gram fresh weight of cells). A K{sub i} of 100 micromolar for 4-hydroxycinnamate: CoA-ligase in a crude preparation was estimated for this inhibitor. It is suggested that the conversion of cinnamic acids into benzoic acids does not involve cinnamoyl CoA esters as intermediates. Feeding of {sup 14}C-cinnamic acid and {sup 14}C-ferulic acid to cells treated with MDCA indicate that cinnamic acid, but not ferulic acid, is a precursor of vanillic acid in these cultivated cells of V. planifolia.},
doi = {10.1104/pp.94.1.95},
journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 94:1,
place = {United States},
year = {1990},
month = {9}
}