Development of a simple device for processing whole blood samples into measured aliquots of plasma
A capillary processor and aliquoter (CPA) has been designed and fabricated that is capable of accepting aliquots of whole blood and automatically processing them into discrete aliquots of plasma. The device consists of two disks, each of which contains 16 individual capillaries and a processing rotor. One of the disks accepts larger capillaries, each of which will hold approx. 100 ..mu..L of whole blood. The second disk, which accepts 2.54-cm-long precision capillaries of varying internal diameter, provides for exact sample volumes ranging from 1 to 10 ..mu..L. The processing rotor consists of 16 individual compartments and chambers to accept both disks. Gravimetric and photometric evaluation of the CPA indicates that it is capable of entraining and delivering microliter volumes of liquids with a degree of precision and accuracy (1 to 2%) approaching that of a state-of-the-art mechanical pipette. In addition, we have demonstrated that aliquots of whole blood can be transferred into the chambers of the processing unit and separated into their cellular and plasma fractions, which can then be analyzed with an acceptable degree of precision (i.e., C.V.s of approx. +-3% for serum enzyme measurements). 15 refs., 6 figs., 4 tbls.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- DOE Contract Number:
- AC05-84OR21400
- OSTI ID:
- 5624628
- Report Number(s):
- CONF-8604197-1; ON: DE86010008
- Resource Relation:
- Conference: 18. annual Oak Ridge conference on advanced analytical concepts for the clinical laboratory, Knoxville, TN, USA, 17 Apr 1986; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
Similar Records
Device and method for automated separation of a sample of whole blood into aliquots
Device and method for automated separation of a sample of whole blood into aliquots
Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
BLOOD CELLS
CENTRIFUGATION
BLOOD PLASMA
CENTRIFUGAL FAST ANALYZERS
ACCURACY
PERFORMANCE
CAPILLARY FLOW
GRAVIMETRIC ANALYSIS
PHOTOMETRY
ROTORS
VISCOSITY
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
CHEMICAL ANALYSIS
FLUID FLOW
MATERIALS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
440300* - Miscellaneous Instruments- (-1989)
400105 - Separation Procedures