Regulation of glucose transport by insulin, bombesin, and bradykinin in Swiss 3T3 fibroblasts: Involvement of protein kinase C-dependent and -independent mechanisms
- Univ. of Milano (Italy)
Glucose transport stimulation by insulin, bombesin, and bradykinin in Swiss 3T3 fibroblasts was compared with the phosphoinositide hydrolysis effects of the same stimulants in a variety of experimental paradigms known to affect generation and/or functioning of intracellular second messengers: short- and long-term treatments with phorbol dibutyrate, that cause activation and down-regulation of protein kinase C, respectively; cell loading with high (quin2), that causes clamping of (Ca{sup 2+}){sub i} near the resting level; poisoning with pertussis toxin, that affects the GTP binding proteins of the Go/Gi class; treatment with Ca{sup 2+} ionophores. ({sup 14}C) glucose transport stimulation by maximal (insulin) was affected by neither pertussis toxin nor protein kinase C down-regulation. This result correlates with the lack of effect of insulin on phosphoinositide hydrolysis. In contrast, part of the glucose transport responses induced by bombesin and bradykinin appeared to be mediated by protein kinase C in proportion with the stimulation induced by these peptides on the phosphoinositide hydrolysis. The protein kinase C-independent portion of the response to bradykinin was found to be inhibitable by pertussis toxin. This latter result might suggest an interaction between the bradykinin receptor and a glucose transporter, mediated by a protein of the Go/Gi class.
- OSTI ID:
- 5623157
- Journal Information:
- Experimental Cell Research; (United States), Vol. 182:1; ISSN 0014-4827
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARBON 14 COMPOUNDS
MEMBRANE TRANSPORT
GLUCOSE
PHOSPHOTRANSFERASES
BIOCHEMISTRY
BRADYKININ
INSULIN
TRITIUM COMPOUNDS
ALDEHYDES
CARBOHYDRATES
CARBON COMPOUNDS
CHEMISTRY
ENZYMES
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
KININS
LABELLED COMPOUNDS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
POLYPEPTIDES
PROTEINS
SACCHARIDES
TRANSFERASES
550301* - Cytology- Tracer Techniques