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Title: (Restriction of virus infection by plants: Annual report, 1986)

Abstract

This research concerns the strong resistance, or even immunity, against a specific virus that is exhibited by one or a few lines of a plant species, in contrast to the general susceptibility of most lines of that species. The contrast between the reactions to virus inoculation of different lines of one species implies that a single gene or a very few genes may mediate the resistance or immunity. The prospects for isolating, studying and transferring such a gene should be good for a system with these characteristics. Seedlings of a line Arlington of the cowpea (Vigna unguiculata) fail to support the replication of cowpea mosaic virus strain SB (CPMV-SB). Genetic crosses of Arlington cowpea to the systemic host Blackeye 5 cowpea show that the immunity is inherited as a simple dominant gene. In contrast to the seedlings, the protoplasts of the Arlington cowpea support CPMV-SB replication, but only to a very low level compared to protoplasts of Blackeye 5 cowpeas. From evidence reported earlier we concluded that Arlington cowpea protoplasts restrict the production of CPMV-SB proteins. We postulated, and obtained evidence for, a proteinase inhibitor that is specific for a CPMV-SB proteinase. This proteinase inhibitor is our prime candidate formore » the mediator of the resistance of Arlington protoplasts to CPMV-SB. Progress to date is described.« less

Authors:
Publication Date:
Research Org.:
California Univ., Davis, CA (United States). Dept. of Plant Pathology
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
5622771
Report Number(s):
DOE/ER/13353-T1
ON: DE92007726
DOE Contract Number:  
FG03-85ER13353
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PLANTS; IMMUNITY; DNA SEQUENCING; ENZYMES; GENES; PROGRESS REPORT; PROTEINS; PROTEOLYSIS; SEEDLINGS; VIRUSES; CHEMICAL REACTIONS; DECOMPOSITION; DOCUMENT TYPES; MICROORGANISMS; ORGANIC COMPOUNDS; PARASITES; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics; 550200 - Biochemistry

Citation Formats

Bruening, G. (Restriction of virus infection by plants: Annual report, 1986). United States: N. p., 1986. Web. doi:10.2172/5622771.
Bruening, G. (Restriction of virus infection by plants: Annual report, 1986). United States. doi:10.2172/5622771.
Bruening, G. Fri . "(Restriction of virus infection by plants: Annual report, 1986)". United States. doi:10.2172/5622771. https://www.osti.gov/servlets/purl/5622771.
@article{osti_5622771,
title = {(Restriction of virus infection by plants: Annual report, 1986)},
author = {Bruening, G.},
abstractNote = {This research concerns the strong resistance, or even immunity, against a specific virus that is exhibited by one or a few lines of a plant species, in contrast to the general susceptibility of most lines of that species. The contrast between the reactions to virus inoculation of different lines of one species implies that a single gene or a very few genes may mediate the resistance or immunity. The prospects for isolating, studying and transferring such a gene should be good for a system with these characteristics. Seedlings of a line Arlington of the cowpea (Vigna unguiculata) fail to support the replication of cowpea mosaic virus strain SB (CPMV-SB). Genetic crosses of Arlington cowpea to the systemic host Blackeye 5 cowpea show that the immunity is inherited as a simple dominant gene. In contrast to the seedlings, the protoplasts of the Arlington cowpea support CPMV-SB replication, but only to a very low level compared to protoplasts of Blackeye 5 cowpeas. From evidence reported earlier we concluded that Arlington cowpea protoplasts restrict the production of CPMV-SB proteins. We postulated, and obtained evidence for, a proteinase inhibitor that is specific for a CPMV-SB proteinase. This proteinase inhibitor is our prime candidate for the mediator of the resistance of Arlington protoplasts to CPMV-SB. Progress to date is described.},
doi = {10.2172/5622771},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1986},
month = {12}
}