Simultaneous development of antibody-dependent cellular cytotoxicity (ADCC) and natural killer (NK) activity in irradiated mice reconstituted with bone marrow cells
Spleen cells from irradiated, bone marrow-reconstituted mice were tested for their ability to mediate antibody-dependent cellular cytotoxicity against P815 target (ADCC-P815), ADCC against sheep red blood cells (ADCC-SRBC), and natural killer (NK) activity judged as YAC-1 lysis at different times after bone marrow reconstitution. Donor-derived ADCC-P815 effectors were found to appear in the spleens 10-12 days after bone marrow reconstitution simultaneously with the appearance of donor-derived NK cells. NK cells recently derived from bone marrow are known to express the Thy-1 antigen; the phenotype of the ''early'' ADCC-P815 effectors was found to be the same as that of NK cells, i.e., Thy-1+, asialo-GM1+. These data suggest that ADCC-P815 effector cells belong to the NK cell population. ADCC-SRBC, in contrast to ADCC-P815 and NK activity, was already high on Day 7 after bone marrow reconstitution. However, it was mediated partly by recipient-derived effectors. ADCC-SRBC effectors were characterized to be different from ADCC-P815 effectors.
- Research Organization:
- Univ. of Helsinki, Finland
- OSTI ID:
- 5621907
- Journal Information:
- Cell. Immunol.; (United States), Vol. 109:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LYMPHOCYTES
LYSIS
SPLEEN CELLS
IRRADIATION
ANTIGEN-ANTIBODY REACTIONS
BONE MARROW CELLS
CELL DIFFERENTIATION
ERYTHROCYTES
IMMUNITY
MICE
RADIATION CHIMERAS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHIMERAS
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MAMMALS
MATERIALS
MOSAICISM
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals