Flow cytometric and radioisotopic determinations of platelet survival time in normal cats and feline leukemia virus-infected cats
This study demonstrates the potential usefulness of a flow cytometric technique to measure platelet survival time in cats utilizing autologous platelets labeled in vitro with fluorescein isothiocyanate (FITC). When compared with a 51Cr method, no significant differences in estimated survival times were found. Both the 51Cr and FITC-labeling procedures induced similar changes in platelet shape and collagen-induced aggregation. Platelets labeled with FITC had significantly greater volumes compared with those of glutaraldehyde-fixed platelets. These changes were primarily related to the platelet centrifugation and washing procedures rather than the labels themselves. This novel technique potentially has wide applicability to cell circulation time studies as flow cytometry equipment becomes more readily available. Problems with the technique are discussed. In a preliminary study of the platelet survival time in feline leukemia virus (FeLV)-infected cats, two of three cats had significantly reduced survival times using both flow cytometric and radioisotopic methods. These data suggest increased platelet turnover in FeLV-infected cats.
- Research Organization:
- Ohio State Univ., Columbus
- OSTI ID:
- 5620498
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BLOOD PLATELETS
SURVIVAL TIME
CELL FLOW SYSTEMS
DIAGNOSTIC USES
CATS
CHROMIUM 51
LEUKEMIA
THIOCYANATES
ANIMALS
ANTITHYROID DRUGS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CHROMIUM ISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HEMIC DISEASES
HORMONE ANTAGONISTS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
MATERIALS
NEOPLASMS
NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
USES
VERTEBRATES
550301* - Cytology- Tracer Techniques