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Title: Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae

Abstract

In Pseudomonas syringae pv. tomato PT23.2, plasmid pPT23A (101 kb) is involved in synthesis of the phytotoxin coronatine. The physical characterization of mutations that abolished coronatine production indicated that at least 30 kb of pPT23A DNA are required for toxin synthesis. In the present study, {sup 32}P-labeled DNA fragments from the 30-kb region of pPT23A hybridized to plasmid DNAs from several coronatine-producing pathovars of P. syringae under conditions of high stringency. These experiments indicated that this region of pPT23A was strongly conserved in large plasmids (90 to 105 kb) that reside in P. syringae pv. atropurpurea, glycinea, and morsprunorum. The functional significance of the observed homology was demonstrated in marker-exchange experiments in which Tn5-inactivated sequences from the 30-kb region of pPT23A were used to mutate coronatine synthesis genes in the three heterologous pathovars. Physical characterization of the Tn5 insertions generated by marker exchange indicated that genes controlling coronatine synthesis in P. syringae pv. atropurpurea 1304, glycinea 4180, and morsprunorum 567 and 3714 were located on the large indigenous plasmids where homology was originally detected. Therefore, coronatine biosynthesis genes are strongly conserved in the plasmid DNAs of four producing pathovars, despite their disparate origins (California, Japan, New Zealand, Great Britain, andmore » Italy).« less

Authors:
;  [1];  [2]
  1. Oklahoma State Univ., Stillwater (United States)
  2. Dept. of Scientific and Industrial Research, Auckland (New Zealand)
Publication Date:
OSTI Identifier:
5620309
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 57:4; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; TOXINS; BIOSYNTHESIS; DNA HYBRIDIZATION; GENES; MUTATIONS; PHOSPHORUS 32; PLASMIDS; TRACER TECHNIQUES; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; HAZARDOUS MATERIALS; HYBRIDIZATION; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MATERIALS; NUCLEI; ODD-ODD NUCLEI; PHOSPHORUS ISOTOPES; RADIOISOTOPES; SYNTHESIS; TOXIC MATERIALS; 550901* - Pathology- Tracer Techniques

Citation Formats

Bender, C L, Young, S A, and Mitchell, R E. Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae. United States: N. p., 1991. Web.
Bender, C L, Young, S A, & Mitchell, R E. Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae. United States.
Bender, C L, Young, S A, and Mitchell, R E. Mon . "Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae". United States.
@article{osti_5620309,
title = {Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae},
author = {Bender, C L and Young, S A and Mitchell, R E},
abstractNote = {In Pseudomonas syringae pv. tomato PT23.2, plasmid pPT23A (101 kb) is involved in synthesis of the phytotoxin coronatine. The physical characterization of mutations that abolished coronatine production indicated that at least 30 kb of pPT23A DNA are required for toxin synthesis. In the present study, {sup 32}P-labeled DNA fragments from the 30-kb region of pPT23A hybridized to plasmid DNAs from several coronatine-producing pathovars of P. syringae under conditions of high stringency. These experiments indicated that this region of pPT23A was strongly conserved in large plasmids (90 to 105 kb) that reside in P. syringae pv. atropurpurea, glycinea, and morsprunorum. The functional significance of the observed homology was demonstrated in marker-exchange experiments in which Tn5-inactivated sequences from the 30-kb region of pPT23A were used to mutate coronatine synthesis genes in the three heterologous pathovars. Physical characterization of the Tn5 insertions generated by marker exchange indicated that genes controlling coronatine synthesis in P. syringae pv. atropurpurea 1304, glycinea 4180, and morsprunorum 567 and 3714 were located on the large indigenous plasmids where homology was originally detected. Therefore, coronatine biosynthesis genes are strongly conserved in the plasmid DNAs of four producing pathovars, despite their disparate origins (California, Japan, New Zealand, Great Britain, and Italy).},
doi = {},
journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 57:4,
place = {United States},
year = {1991},
month = {4}
}