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Title: Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product

Abstract

The aberrant overexpression of interleukin 6 (IL-6) is implicated as an autocrine mechanism in the enhanced proliferation of the neoplastic cell elements in various B- and T-cell malignancies and in some carcinomas and sarcomas; many of these neoplasms have been shown to be associated with a mutated p53 gene. The possibility that wild-type (wt) p53, a nuclear tumor-suppressor protein, but not its transforming mutants might serve to repress IL-6 gene expression was investigated in HeLa cells. The authors transiently cotransfected these cells with constitutive cytomegalovirus (CMV) enhancer/promoter expression plasmids overproducing wt or mutant human or murine p53 and with appropriate chloramphenicol acetyltransferase (CAT) reporter plasmids containing the promoter elements of human IL-6, c-fos, or {beta}-actin genes or of porcine major histocompatibility complex (MHC) class I gene in pN-38 to evaluate the effect of the various p53 species on these promoters. These observations identify transcriptional repression as a property of p53 and suggest that p53 and RB may be involved as transcriptional repressors in modulating IL-6 gene expression during cellular differentiation and oncogenesis.

Authors:
; ;  [1]
  1. Rockefeller Univ., New York, NY (United States)
Publication Date:
OSTI Identifier:
5604146
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:17; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENE REPRESSORS; TRANSCRIPTION; LYMPHOKINES; GENE REGULATION; ACTIN; CARCINOGENESIS; CELL DIFFERENTIATION; HELA CELLS; HISTOCOMPATIBILITY COMPLEX; MYCELIUM; SULFUR 35; TUMOR CELLS; ANIMAL CELLS; ANTIGENS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; GROWTH FACTORS; ISOTOPES; LIGHT NUCLEI; MITOGENS; NUCLEI; NUCLEOPROTEINS; ORGANIC COMPOUNDS; PATHOGENESIS; PLANT TISSUES; PROTEINS; RADIOISOTOPES; SULFUR ISOTOPES; TISSUES; 550200* - Biochemistry

Citation Formats

Santhanam, U, Ray, A, and Sehgal, P B. Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product. United States: N. p., 1991. Web. doi:10.1073/pnas.88.17.7605.
Santhanam, U, Ray, A, & Sehgal, P B. Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product. United States. https://doi.org/10.1073/pnas.88.17.7605
Santhanam, U, Ray, A, and Sehgal, P B. 1991. "Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product". United States. https://doi.org/10.1073/pnas.88.17.7605.
@article{osti_5604146,
title = {Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product},
author = {Santhanam, U and Ray, A and Sehgal, P B},
abstractNote = {The aberrant overexpression of interleukin 6 (IL-6) is implicated as an autocrine mechanism in the enhanced proliferation of the neoplastic cell elements in various B- and T-cell malignancies and in some carcinomas and sarcomas; many of these neoplasms have been shown to be associated with a mutated p53 gene. The possibility that wild-type (wt) p53, a nuclear tumor-suppressor protein, but not its transforming mutants might serve to repress IL-6 gene expression was investigated in HeLa cells. The authors transiently cotransfected these cells with constitutive cytomegalovirus (CMV) enhancer/promoter expression plasmids overproducing wt or mutant human or murine p53 and with appropriate chloramphenicol acetyltransferase (CAT) reporter plasmids containing the promoter elements of human IL-6, c-fos, or {beta}-actin genes or of porcine major histocompatibility complex (MHC) class I gene in pN-38 to evaluate the effect of the various p53 species on these promoters. These observations identify transcriptional repression as a property of p53 and suggest that p53 and RB may be involved as transcriptional repressors in modulating IL-6 gene expression during cellular differentiation and oncogenesis.},
doi = {10.1073/pnas.88.17.7605},
url = {https://www.osti.gov/biblio/5604146}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:17,
place = {United States},
year = {Sun Sep 01 00:00:00 EDT 1991},
month = {Sun Sep 01 00:00:00 EDT 1991}
}