Evidence for a single class of somatostatin receptors in ground squirrel cerebral cortex

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Peptides (Fayetteville, N.Y.); (United States)

DOI:https://doi.org/10.1016/0196-9781(89)90076-4· OSTI ID:5603874

Krantic, S; Petrovic, V M; Quirion, R; Kordon, C

In the present study we characterized high-affinity somatostatin (SRIF) binding sites (Kd = 2.06 +/- 0.32 nM and Bmax = 295 +/- 28 fmol/mg protein) in cerebral cortex membrane preparations of European ground squirrel using /sup 125/I-(Tyr0-D-Trp8)-SRIF14 as a radioligand. The inhibition of radioligand specific binding by SRIF14, as well as by its agonists (SRIF28, Tyr0-D-Trp8-SRIF14, SMS 201 995) was complete and monophasic, thus revealing a single population of somatostatinergic binding sites. Radioautographic analysis of /sup 125/I-(Tyr0-D-Trp8)-SRIF14 labeled brain sections confirmed the results of our biochemical study. The homogeneity of SRIF binding sites in the ground squirrel neocortex was not dependent on the animal's life-cycle phase.

• Research Organization: Institut for Biological Research Dr S. Stankovic, Beograd (Yugoslavia)
• OSTI ID: 5603874
• Journal Information: Peptides (Fayetteville, N.Y.); (United States), Vol. 10:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SOMATOSTATIN
RECEPTORS
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
CEREBRAL CORTEX
HIBERNATION
IODINE 125
LIGANDS
SQUIRRELS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
BRAIN
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CEREBRUM
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques