Characterization of conformational features of DNA heteroduplexes containing aldehyde abasic sites

Journal Article · Tue Oct 15 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00105a017· OSTI ID:5601931

Withka, J M; Wilde, J A; Bolton, P H ^[1]; Mazumder, A; Gerlt, J A ^[2]

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1. Wesleyan Univ., Middletown, CT (United States)
2. Univ. of Maryland, College Park (United States)

The DNA duplexes shown below, with D indicating deoxyribose aldehyde abasic sites and numbering from 5{prime} to 3{prime}, have been investigated by NMR. The {sup 31}P-{sup 1}H correlation data indicate that the backbones of these duplex DNAs are regular. One- and two-dimensional {sup 1}H NMR data indicate that the duplexes are right-handed and B-form. Conformational changes due to the presence of the abasic site extend to the two base pairs adjacent to the lesion site with the local conformation of the DNA being dependent on whether the abasic site is in the {alpha} or {beta} configuration. The aromatic base of residue A17 in the position opposite the abasic site is predominantly stacked i the helix as is G17 in the analogous sample. Imino lifetimes of the AT base pairs are much longer in samples with an abasic site than in those containing a Watson-Crick base pair. The conformational and dynamical properties of the duplex DNAs containing the naturally occurring aldehyde abasic site are different from those of duplex DNAs containing a variety of analogues of the abasic site.

• OSTI ID: 5601931
• Journal Information: Biochemistry; (United States), Vol. 30:41; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

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PROTONS
BARYONS
ELEMENTARY PARTICLES
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