Stretches of oligogalacturonan are present in the backbone of rhamnogalacturonan I from cotton suspension culture cell walls
- Oklahoma State Univ., Stillwater (United States)
A polysaccharide fraction, solubilized from cotton suspension culture cell walls by sequential treatments by endopolygalacturonase and cellulase and purified by gel filtration chromatography, contained the sugars characteristic of rhamnogalacturonan I (RGI). A known structural feature of the backbone of RGI is the repeating disaccharide galA-rha; however, the ratio of galA to rha residues was found to be 1 {center dot} 9:1. Treatment of the RGI preparation with liquid HF (containing 1% water) at {minus}23{degree} caused cleavage of most of the rhamnosyl linkages without cleavage of galacturonosyl linkages. Neutral sugar linkages of the sidechains were also mostly cleaved. Characterization of the products from the backbone by nmr spectroscopy, compositional analysis, and liquid secondary ion mass spectrometry suggested that the backbone of the RGI is made up of short segments ({approximately} 10 units) of the galA-rha repeat interspersed with short homogalacturonan segments of {approximately} 10 galA residues of which about 40% are methyl esterified. The sequential enzyme treatments only solubilize {approximately} 40% of the RGI. The residual RGI contains a higher proportion of falA-rha repeats in its backbone.
- DOE Contract Number:
- FG05-86ER13496
- OSTI ID:
- 5601021
- Report Number(s):
- CONF-9107184-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (United States), Vol. 96:1; Conference: Annual meeting of the American Society of Plant Physiology, Albuquerque, NM (United States), 28 Jul - 1 Aug 1991; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL WALL
CHEMICAL COMPOSITION
COTTON PLANTS
PHYSIOLOGY
CELL CULTURES
CELLULASE
POLYSACCHARIDES
CARBOHYDRATES
CELL CONSTITUENTS
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
PROTEINS
SACCHARIDES
551000* - Physiological Systems