Sixty-five radiation hybrids for the short arm of human chromosome 6: their value as a mapping panel and as a source for rapid isolation of new probes using repeat element-mediated PCR
- Department of Pediatrics, Baylor College of Medicine, Houston, Tx (USA)
We have used an irradiation and fusion procedure to generate somatic cell hybrids that retain fragments of the short arm of human chromosome 6 (6p). To identify hybrids retaining human material, we performed repeat element-mediated PCR on crude lysates of cells from individual clones. Sixty-five hybrids were shown to contain human material and fifty of those contained one or more 6p-specific probes. Detailed characterization of these hybrids identified a subset that divides 6p into ten mapping intervals. Using repeat element-mediated PCR, we were able to isolate and map 61 new DNA fragments from specific regions of 6p. Fifteen of these fragments were used to screen for restriction fragment length polymorphisms (RFLPs), and nine identified RFLPs with one or more enzymes. The radiation hybrids described in this study provide a valuable resource for high-resolution mapping of 6p and for the rapid isolation of region-specific markers.
- OSTI ID:
- 5592637
- Journal Information:
- Genomics; (United States), Vol. 9:4; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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DNA
BIOASSAY
SOMATIC CELLS
GENETIC MAPPING
AMINO ACID SEQUENCE
BIOLOGICAL MARKERS
CHROMOSOMES
GAMMA RADIATION
GENETIC RADIATION EFFECTS
MAN
POLYMERASES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
ENZYMES
GENETIC EFFECTS
IONIZING RADIATIONS
MAMMALS
MAPPING
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
RADIATION EFFECTS
RADIATIONS
TRANSFERASES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture