Altered metabolic incorporation of fucose and leucine into PNS myelin of 25-week-old diabetic (C57BL/Ks (db/db)) mice: effects of untreated diabetes on nerve metabolism
Sciatic nerves of 25-week-old genetically diabetic (C57BL/Ks (db/db)) mice and their litter-mate controls were removed, and their metabolic incorporation of (/sup 3/H)fucose and (/sup 14/C)leucine into myelin was studied in vitro. Untreated diabetic animals showed significant increases (p less than 0.05) in the fucose/leucine incorporation into myelin when compared to values found for their litter-mates. These results correlated well with previous experiments performed on alloxan or streptozotocin-diabetic rats and thus show the in vitro incubation procedure to be a good indicator of altered metabolic conditions in peripheral nerves due to diabetes mellitus. The resulting ratio increases seen in diabetic animals is at variance with the decrease in ratios found in animals undergoing typical Wallerian degeneration. These results suggest that different metabolic processes operate in untreated diabetics than in normals or in those undergoing other degenerative nerve processes.
- Research Organization:
- Department of Anatomy, Indiana University School of Medicine, Indianapolis
- OSTI ID:
- 5590285
- Journal Information:
- Neurochem. Res.; (United States), Vol. 8:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIABETES MELLITUS
BIOLOGICAL MODELS
PATHOLOGICAL CHANGES
HEXOSES
METABOLISM
LEUCINE
MYELIN
LABELLING
PROTEINS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
DOUBLE LABELLING
MICE
SCIATIC NERVE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACIDS
DISEASES
ENDOCRINE DISEASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIPOPROTEINS
MAMMALS
METABOLIC DISEASES
MONOSACCHARIDES
NERVES
NERVOUS SYSTEM
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
SACCHARIDES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques