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Title: Investigation of metabolites and optimization of bacterial culture for coal biogasification Phase 1. Topical report, February 1989-December 1990

Abstract

ARCTECH is developing a novel coal biogasification approach using microbial consortia. The objective of the study was to obtain a fundamental understanding of the pathways utilized during bioconversion of coals to methane and define functional relationships among microbes which mediate the biogasification process. Three coals were biosolubilized using an aerobic mixed culture and the solubilized coal products were separated by ultrafiltration into seven fractions based on molecular weight. The fractions were subjected to chemical analysis and used as substrates for biogasification. Methane production was enhanced through culture manipulation and adaptation. Maximum methane production was achieved with the 3-10 K fraction of premium Beulah lignite (571 cc/g of coal carbon). Microscopic observation of the cultures revealed that different morphological types of bacteria predominated in each. These differences can be attributed to the selective pressure exerted by the various coal fractions present as principal carbon sources. Pyruvic, oxalic and tartaric acids were identified as key intermediates resulting from biogasification of these fractions. Changes in coal functional groups resulted from biogasification. By using soluble coal fractions as biogasification model compounds, ARCTECH identified several intermediates in the biogasification pathway, detected chemical changes in soluble carbon substrates effected by microbial consortia and developed cultures formore » biological conversion of coal to methane.« less

Authors:
; ;
Publication Date:
Research Org.:
ARCTECH, Inc., Alexandria, VA (United States)
OSTI Identifier:
5587330
Alternate Identifier(s):
OSTI ID: 5587330
Report Number(s):
PB-92-137595/XAB
CNN: GRI-5088-221-1756
Resource Type:
Technical Report
Resource Relation:
Other Information: Sponsored by Gas Research Inst., Chicago, IL
Country of Publication:
United States
Language:
English
Subject:
01 COAL, LIGNITE, AND PEAT; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BACTERIA; PERFORMANCE TESTING; COAL; BIODEGRADATION; METHANE; BIOSYNTHESIS; AEROBIC CONDITIONS; AIR POLLUTION ABATEMENT; BIOCHEMISTRY; BIOLOGICAL PATHWAYS; CHEMICAL ANALYSIS; COAL GASIFICATION; CULTURE MEDIA; DISSOLUTION; LIGNITE; METABOLITES; MICROORGANISMS; MIXTURES; MOLECULAR WEIGHT; MORPHOLOGY; REACTION INTERMEDIATES; ULTRAFILTRATION; ALKANES; BROWN COAL; CARBONACEOUS MATERIALS; CHEMICAL REACTIONS; CHEMISTRY; DECOMPOSITION; DISPERSIONS; ENERGY SOURCES; FILTRATION; FOSSIL FUELS; FUELS; GASIFICATION; HYDROCARBONS; MATERIALS; ORGANIC COMPOUNDS; POLLUTION ABATEMENT; SEPARATION PROCESSES; SYNTHESIS; TESTING; THERMOCHEMICAL PROCESSES 010404* -- Coal, Lignite, & Peat-- Gasification; 560300 -- Chemicals Metabolism & Toxicology

Citation Formats

Barik, S., Tiemens, K., and Isbister, J. Investigation of metabolites and optimization of bacterial culture for coal biogasification Phase 1. Topical report, February 1989-December 1990. United States: N. p., 1991. Web.
Barik, S., Tiemens, K., & Isbister, J. Investigation of metabolites and optimization of bacterial culture for coal biogasification Phase 1. Topical report, February 1989-December 1990. United States.
Barik, S., Tiemens, K., and Isbister, J. Fri . "Investigation of metabolites and optimization of bacterial culture for coal biogasification Phase 1. Topical report, February 1989-December 1990". United States.
@article{osti_5587330,
title = {Investigation of metabolites and optimization of bacterial culture for coal biogasification Phase 1. Topical report, February 1989-December 1990},
author = {Barik, S. and Tiemens, K. and Isbister, J.},
abstractNote = {ARCTECH is developing a novel coal biogasification approach using microbial consortia. The objective of the study was to obtain a fundamental understanding of the pathways utilized during bioconversion of coals to methane and define functional relationships among microbes which mediate the biogasification process. Three coals were biosolubilized using an aerobic mixed culture and the solubilized coal products were separated by ultrafiltration into seven fractions based on molecular weight. The fractions were subjected to chemical analysis and used as substrates for biogasification. Methane production was enhanced through culture manipulation and adaptation. Maximum methane production was achieved with the 3-10 K fraction of premium Beulah lignite (571 cc/g of coal carbon). Microscopic observation of the cultures revealed that different morphological types of bacteria predominated in each. These differences can be attributed to the selective pressure exerted by the various coal fractions present as principal carbon sources. Pyruvic, oxalic and tartaric acids were identified as key intermediates resulting from biogasification of these fractions. Changes in coal functional groups resulted from biogasification. By using soluble coal fractions as biogasification model compounds, ARCTECH identified several intermediates in the biogasification pathway, detected chemical changes in soluble carbon substrates effected by microbial consortia and developed cultures for biological conversion of coal to methane.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {11}
}

Technical Report:
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