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Title: Vasoactive intestinal peptide stimulates tracheal submucosal gland secretion in ferret

Abstract

We studied the effect of vasoactive intestinal peptide (VIP) on the output of 35S-labeled macromolecules from ferret tracheal explants either placed in beakers or suspended in modified Ussing chambers. In Ussing chamber experiments, the radiolabel precursor, sodium (35S)sulfate, and all drugs were placed on the submucosal side of the tissue. Washings were collected at 30-min intervals from the luminal side and were dialyzed to remove unbound 35S, leaving radiolabeled macromolecules. Vasoactive intestinal peptide at 3 X 10(-7) M stimulated bound 35S output by a mean of + 252.6% (n . 14). The VIP response was dose-dependent with a near maximal response and a half maximal response at approximately 10(-6) M and 10(-8), M, respectively. The VIP effect was not inhibited by a mixture of tetrodotoxin, atropine, I-propranolol, and phentolamine. Vasoactive intestinal peptide had no effect on the electrical properties of the of the tissues. We conclude that VIP stimulates output of sulfated-macromolecules from ferret tracheal submucosal glands without stimulating ion transport. Our studies also suggest that VIP acts on submucosal glands via specific VIP receptors. Vasoactive intestinal peptide has been shown to increase intracellular levels of cyclic AMP, and we suggest that this may be the mechanism for its effectmore » on the output of macromolecules. This mechanism may be important in the neural regulation of submucosal gland secretion.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Cardiovascular Research Institute, University of California, San Francisco
OSTI Identifier:
5586656
Resource Type:
Journal Article
Journal Name:
Am. Rev. Respir. Dis.; (United States)
Additional Journal Information:
Journal Volume: 128:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; TRACHEA; METABOLIC ACTIVATION; INTESTINES; MAMMALS; MUCOUS MEMBRANES; PEPTIDES; STIMULATION; SULFUR 35; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; EVEN-ODD NUCLEI; GASTROINTESTINAL TRACT; ISOTOPES; LIGHT NUCLEI; MEMBRANES; NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RESPIRATORY SYSTEM; SULFUR ISOTOPES; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Peatfield, A.C., Barnes, P.J., Bratcher, C., Nadel, J.A., and Davis, B. Vasoactive intestinal peptide stimulates tracheal submucosal gland secretion in ferret. United States: N. p., 1983. Web. doi:10.1164/arrd.1983.128.1.89.
Peatfield, A.C., Barnes, P.J., Bratcher, C., Nadel, J.A., & Davis, B. Vasoactive intestinal peptide stimulates tracheal submucosal gland secretion in ferret. United States. doi:10.1164/arrd.1983.128.1.89.
Peatfield, A.C., Barnes, P.J., Bratcher, C., Nadel, J.A., and Davis, B. Fri . "Vasoactive intestinal peptide stimulates tracheal submucosal gland secretion in ferret". United States. doi:10.1164/arrd.1983.128.1.89.
@article{osti_5586656,
title = {Vasoactive intestinal peptide stimulates tracheal submucosal gland secretion in ferret},
author = {Peatfield, A.C. and Barnes, P.J. and Bratcher, C. and Nadel, J.A. and Davis, B.},
abstractNote = {We studied the effect of vasoactive intestinal peptide (VIP) on the output of 35S-labeled macromolecules from ferret tracheal explants either placed in beakers or suspended in modified Ussing chambers. In Ussing chamber experiments, the radiolabel precursor, sodium (35S)sulfate, and all drugs were placed on the submucosal side of the tissue. Washings were collected at 30-min intervals from the luminal side and were dialyzed to remove unbound 35S, leaving radiolabeled macromolecules. Vasoactive intestinal peptide at 3 X 10(-7) M stimulated bound 35S output by a mean of + 252.6% (n . 14). The VIP response was dose-dependent with a near maximal response and a half maximal response at approximately 10(-6) M and 10(-8), M, respectively. The VIP effect was not inhibited by a mixture of tetrodotoxin, atropine, I-propranolol, and phentolamine. Vasoactive intestinal peptide had no effect on the electrical properties of the of the tissues. We conclude that VIP stimulates output of sulfated-macromolecules from ferret tracheal submucosal glands without stimulating ion transport. Our studies also suggest that VIP acts on submucosal glands via specific VIP receptors. Vasoactive intestinal peptide has been shown to increase intracellular levels of cyclic AMP, and we suggest that this may be the mechanism for its effect on the output of macromolecules. This mechanism may be important in the neural regulation of submucosal gland secretion.},
doi = {10.1164/arrd.1983.128.1.89},
journal = {Am. Rev. Respir. Dis.; (United States)},
number = ,
volume = 128:1,
place = {United States},
year = {1983},
month = {7}
}