Estradiol target neurons in the hypothalamic arcuate nucleus and lateral ventromedial nucleus of young adult, reproductively senescent, and monosodium glutamate-lesioned female golden hamsters

Journal Article · Thu Sep 01 00:00:00 EDT 1983 · J. Gerontol.; (United States)

DOI:https://doi.org/10.1093/geronj/38.5.532· OSTI ID:5578569

Blaha, G C; Lamperti, A A

Histoautoradiographic methods were used to assess estrogen target neurons in the hypothalamic arcuate nucleus (ARC) and ventromedial nucleus, lateral portion (LVM), comparing young adult and aged female golden hamsters. A subgroup of young adult females had ARC lesions induced by monosodium glutamate at neonatal day 8. All were ovariectomized to remove endogenous estrogens. Controls were given nonradioactive estradiol. After /sup 3/H-estradiol (/sup 3/H-E2) was injected intravenously, hypothalami were removed, frozen, and processed for histoautoradiography. In the ARC and LVM the ratio of /sup 3/H-E2 labelled neurons to total neurons counted was significantly lower in the older animals. Young females with ARC lesions had very few /sup 3/H-E2 labelled neurons remaining in the ARC but had a normal complement in the LVM. Although /sup 3/H-E2 labelled ARC neurons were notably decreased in old females, those ARC neurons that were labelled in the old had virtually the same frequency distribution of the labelling index as in the young, suggesting no change in the average estrogen uptake per target cell.

• Research Organization: Department of Anatomy, University of Cincinnati College of Medicine, OH
• OSTI ID: 5578569
• Journal Information: J. Gerontol.; (United States), Vol. 38:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ESTRADIOL
RECEPTORS
HYPOTHALAMUS
AUTORADIOGRAPHY
NERVE CELLS
CASTRATION
HAMSTERS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
ESTRANES
ESTROGENS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEDICINE
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SURGERY
VERTEBRATES
550201* - Biochemistry- Tracer Techniques