Psoralen plus near-ultraviolet light: a possible new method for measuring DNA repair synthesis
- Nuclear Research Center, Negev, Israel
A new method is proposed to inhibit semiconservative DNA synthesis in cultured cells while DNA repair synthesis is being measured. The cells are treated with the DNA-crosslinking agent Trioxalen (4,5,8-trimethylpsoralen) plus near-ultraviolet light, and consequently 99.5% inhibition of replicative DNA synthesis is achieved. Additional DNA-damaging agents induce thymidine incorporation into the double-stranded regions of the DNA. The new method gave results very similar to those obtained with the benzoylated naphthoylated DEAE (BND) cellulose method using three human fibroblast strains, of which one had deficient capacity for DNA repair synthesis following treatment with ..gamma.. rays and methyl methanesulfonate. The advantages of the new method are simplicity and rapidity, as well as the high extent to which replicative DNA synthesis is inhibited.
- OSTI ID:
- 5578080
- Journal Information:
- Radiat. Res.; (United States), Vol. 95:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
DNA
BIOLOGICAL REPAIR
DNA REPLICATION
BIOLOGICAL RADIATION EFFECTS
FIBROBLASTS
GAMMA RADIATION
MAN
NEAR ULTRAVIOLET RADIATION
PSORALEN
SKIN
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
CONNECTIVE TISSUE CELLS
COUMARINS
DRUGS
ELECTROMAGNETIC RADIATION
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
IONIZING RADIATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SOMATIC CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)
560121 - Radiation Effects on Cells- External Source- (-1987)
550201 - Biochemistry- Tracer Techniques