Kinetic studies of amylase and biomass production by Calvatia gigantea
Production of alpha-amylase (alpha-4, glucan 4-glucanohydrolase, EC 3.2.1.1) by microorganisms has been practiced for many years in small and large scale operations and the literature on this enzyme is voluminous. Aspergillus niger and Aspergillus oryzae have been reported as the main fungal species used for commercial production of the enzyme. On the other hand, large volumes of low-cost agricultural products such as acorn (the perisperm-free dry seed contains approximately 60% starch) are wasted in many countries and provide a challenge to biotechnology to efficiently utilize these rich sources of starch for the production of high added value products like enzymes. C. gigantea is an edible puffball excreting high levels of alpha-amylase when cultivated on different sources of starch containing elevated quantities of toxic tannic compounds. This fungus has been employed for the production of microbial protein from wastes and acorns containing high levels of toxic tannic compounds. The same fungus was also reported to grow on both hydrolyzable and condensed tannins as sole carbon sources. The present work was undertaken to investigate certain kinetic characteristics of alpha-amylase and biomass production by C. gigantea grown on soluble and acorn starch in a lab fermenter. (Refs. 18).
- Research Organization:
- Nuclear Research Center ''DEMOCRITOS'', Aghia Paraskevi, Attiki 15 310, Greece. Biology Division
- OSTI ID:
- 5564674
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 30:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMYLASE
BIOCHEMICAL REACTION KINETICS
SEEDS
BIODEGRADATION
STARCH
TANNIC ACID
AGRICULTURAL WASTES
BENCH-SCALE EXPERIMENTS
FUNGI
AROMATICS
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
KINETICS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC WASTES
PHENOLS
PLANTS
POLYPHENOLS
POLYSACCHARIDES
REACTION KINETICS
REAGENTS
SACCHARIDES
WASTES
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)