Nuclear AP/sub 4/A-binding activity of sea urchin embryos changes in relation to the initiation of S phase
The AP/sub 4/A-binding activity of sea urchin embryos was studied using radioactively labelled diadenosine 5', 5'''-P/sup 1/,P/sup 4/-tetraphosphate (Ap/sub 4/A). Among various subcellular components that can bind (/sup 3/H)AP/sub 4/A, nuclei alone showed the highly specific Ap/sub 4/A-binding activity which was not influenced by the presence of AP/sub 4/A, AP/sub 5/A and GP/sub 4/G. The addition of an excess amount of ATP only slightly reduced the binding of (/sup 3/H)AP/sub 4/A to the nuclei. It was found that AP/sub 4/A binds to the residual proteinaceous structure of nuclei which was resistant to the extraction with 2 M NaCl. The nuclear AP/sub 4/A-binding activity fluctuated cyclically during each cell cycle, with at transient increase at the beginning of S phase followed by an abrupt-decrease within 10 min. When the initiation of S phase was blocked, the increase in the AP/sub 4/A-binding activity was also prevented. It seems that the binding of AP/sub 4/A to the nuclear structural protein is involved in the initiation of S phase.
- Research Organization:
- Univ. of Tokyo, Japan
- OSTI ID:
- 5563650
- Journal Information:
- Exp. Cell Res.; (United States), Vol. 165
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SEA URCHINS
CELL DIVISION
NUCLEOPROTEINS
ADP
CELL CYCLE
CELL NUCLEI
LABELLED COMPOUNDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
AQUATIC ORGANISMS
CELL CONSTITUENTS
ECHINODERMS
INVERTEBRATES
ISOTOPE APPLICATIONS
NUCLEOTIDES
ORGANIC COMPOUNDS
PROTEINS
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques