Glycosphingolipids: sup 2 H NMR study of the influence of carbohydrate headgroup structure on ceramide acyl chain behavior in glycolipid-phospholipid bilayers

Journal Article · Tue May 07 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00232a019· OSTI ID:5556299

Fenske, D B; Jarrell, H C ^[1]; Hamilton, K; Florio, E; Barber, K R; Grant, C W.M. ^[2]

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1. National Research Council of Canada, Ottawa, Ontario (Canada)
2. Univ. of Western Ontario, London (Canada)

Galactosyl- and glucosylceramide, globoside, and dihydrolactosylceramide, bearing (2,2-{sup 2}H{sub 2})stearic acid, have been studied at a concentration of 10 mol % in bilayers of dimyristoylphosphatidylcholine by {sup 2}H NMR. The quadrupolar splittings {Delta}v{sub Q} of the C2 deuterons were measured at several temperatures in the range of 30-60{degree}C. Spin-lattice relaxation times T{sub 1} of C2 deuterons were determined in the same temperature range for all lipids but globoside. T{sub 1} values for the GlcCer and GalCer systems increased with temperature, indicating that the motions responsible for relaxation were in the short correlation time regime. T{sub 1} for deuterons at the acyl chain C2-position of LaCer was observed to decrease with increasing temperature, indicating that the motion(s) dominating relaxation are in the long correlation time regime. Thus the mobility of the acyl chain at the 2-position is reduced in the LacCer with respect to GlcCer and GalCer.

• OSTI ID: 5556299
• Journal Information: Biochemistry; (United States), Vol. 30:18; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
GLYCOLIPIDS
NUCLEAR MAGNETIC RESONANCE
MEMBRANES
MOLECULAR STRUCTURE
CHEMICAL SHIFT
DEUTERIUM COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 31
RELAXATION TIME
SPHINGOMYELINS
SPIN-LATTICE RELAXATION
TEMPERATURE EFFECTS
CARBOHYDRATES
ESTERS
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RELAXATION
RESONANCE
SACCHARIDES
STABLE ISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics