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Title: Influence of volatile anesthetics on muscarinic receptor adenylate cyclase coupling in brain and heart

Abstract

In the present study, the influence of four volatile anesthetics (enflurane, isoflurane, diethyl ether, and chloroform) on (1) muscarinic receptor binding parameters and (2) muscarnic regulation of adenylate cyclase activity was examined using membranes isolated from rat brain and heart. Membranes were equilibrated with each of the four anesthetics for 30 minutes and then during the binding assay. The data obtained can be summarized as follows: (1) volatile anesthetics increased receptor affinity for a radiolabeled antagonists, ({sup 3}H)N-methylscopolamine (({sup 3}H)MS), by decreasing its rate of dissociation in brain stem, but not in cardiac, membranes, (2) volatile anesthetics decreased high affinity ({sup 3}H)Oxotremorine-M binding, (3) volatile anesthetics depressed or eliminated the guanine nucleotide sensitivity of agonist binding. The influence of volatile anesthetics on muscarinic regulation of adenylate cyclase enzyme activity was studied using {alpha}({sup 32}P)ATP as the substrate.

Authors:
Publication Date:
Research Org.:
Medical Coll. of Georgia, Augusta, GA (USA)
OSTI Identifier:
5547742
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANESTHETICS; BIOCHEMICAL REACTION KINETICS; CYCLASES; ENZYME ACTIVITY; SYMPATHOMIMETICS; RECEPTORS; ATP; BRAIN; CELL MEMBRANES; HEART; PHOSPHORUS 32; RATS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; VOLATILE MATTER; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM; CENTRAL NERVOUS SYSTEM DEPRESSANTS; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; LYASES; MAMMALS; MATTER; MEMBRANE PROTEINS; MEMBRANES; NERVOUS SYSTEM; NUCLEI; NUCLEOTIDES; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Anthony, B.L. Influence of volatile anesthetics on muscarinic receptor adenylate cyclase coupling in brain and heart. United States: N. p., 1988. Web.
Anthony, B.L. Influence of volatile anesthetics on muscarinic receptor adenylate cyclase coupling in brain and heart. United States.
Anthony, B.L. Fri . "Influence of volatile anesthetics on muscarinic receptor adenylate cyclase coupling in brain and heart". United States.
@article{osti_5547742,
title = {Influence of volatile anesthetics on muscarinic receptor adenylate cyclase coupling in brain and heart},
author = {Anthony, B.L.},
abstractNote = {In the present study, the influence of four volatile anesthetics (enflurane, isoflurane, diethyl ether, and chloroform) on (1) muscarinic receptor binding parameters and (2) muscarnic regulation of adenylate cyclase activity was examined using membranes isolated from rat brain and heart. Membranes were equilibrated with each of the four anesthetics for 30 minutes and then during the binding assay. The data obtained can be summarized as follows: (1) volatile anesthetics increased receptor affinity for a radiolabeled antagonists, ({sup 3}H)N-methylscopolamine (({sup 3}H)MS), by decreasing its rate of dissociation in brain stem, but not in cardiac, membranes, (2) volatile anesthetics decreased high affinity ({sup 3}H)Oxotremorine-M binding, (3) volatile anesthetics depressed or eliminated the guanine nucleotide sensitivity of agonist binding. The influence of volatile anesthetics on muscarinic regulation of adenylate cyclase enzyme activity was studied using {alpha}({sup 32}P)ATP as the substrate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1988},
month = {1}
}

Thesis/Dissertation:
Other availability
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