Relationship of changing delta 4-steroid 5 alpha-reductase activity to (125I)iododeoxyuridine uptake during regeneration of involuted rat prostates
- Tokyo Medical and Dental Univ. (Japan)
To elucidate the phenotypic expression of proliferating prostatic cells, rats were castrated, and the regenerating process of involuted ventral prostates during testosterone propionate (TP) administration was investigated by examining morphology, (5-{sup 125}I)iododeoxyuridine ({sup 125}I-UdR) uptake, DNA content, weight, acid phosphatase, and delta 4-steroid 5 alpha-reductase (5 alpha-reductase) activities. Morphologically, TP treatment initially increased the number of epithelial cells lining glandular lobules and subsequently restored the shape of epithelial cells. {sup 125}I-UdR uptake peaked on Day 3 of TP treatment and stayed at higher levels than for uncastrated controls until Day 14 of treatment. Prostatic weight, protein content, acid phosphatase, and DNA content returned to uncastrated control levels by Day 14 of TP treatment. TP administration markedly stimulated prostatic 5 alpha-reductase activity, which peaked on the Day 5 of treatment and decreased to uncastrated control levels by Day 14 of treatment. It is concluded that TP administration to castrated rats initially induced active mitotic division of the remaining stem cells, followed by formation of differentiated functional epithelial cells. Prostatic 5 alpha-reductase was highly active at the initial phase of active mitotic cell division. The major portion of the increased enzyme activity can be regarded as a phenotypic expression of stem or transient cells of prostatic epithelium.
- OSTI ID:
- 5547626
- Journal Information:
- Biology of Reproduction; (USA), Vol. 40:4; ISSN 0006-3363
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACID PHOSPHATASE
ENZYME ACTIVITY
OXIDOREDUCTASES
PROSTATE
BIOLOGICAL REGENERATION
STEM CELLS
CELL PROLIFERATION
TESTOSTERONE
BIOCHEMICAL REACTION KINETICS
DNA
EPITHELIUM
IODINE 125
IODODEOXYURIDINE
PHENOTYPE
RATS
TRACER TECHNIQUES
WEIGHT
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIMETABOLITES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
BODY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERASES
GLANDS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROLASES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
IODOURACILS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
KINETICS
MALE GENITALS
MAMMALS
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHATASES
PYRIMIDINES
RADIOISOTOPES
REACTION KINETICS
RECOVERY
RIBOSIDES
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
TISSUES
URACILS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques