High-resolution NMR studies of fibrinogen-like peptides in solution: Structural basis for the bleeding disorder caused by a single mutation of Gly(12) to Val(12) in the A. alpha. chain of human fibrinogen Rouen

Journal Article · Tue Apr 04 00:00:00 EDT 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00433a054· OSTI ID:5546053

Ni, F; Konishi, Y; Bullock, L D; Rivetna, M N; Scheraga, H A ^[1]

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1. Cornell Univ., Ithaca, NY (USA)

In human fibrinogen Rouen, which is the origin of a bleeding disorder, a single amino acid is mutated from Gly(12) to Val(12) in the A{alpha} Chain. In the previous paper of this series, this mutation was predicted to disrupt the structure of fibrinogen-like peptides bound to bovine thrombin. The structural basis of this bleeding disorder has been further assessed by studying the interaction of the following Val(12)-substituted human fibrinogen-like peptides with bovine thrombin in aqueous solution by use of two-dimensional NMR spectroscopy (including TRNOE): Ala-Asp-Ser-Gly-Glu-Gly-Asp(7)-Phe-Leu-Ala-Glu-Val(12)-Gly-Gly-Val-Arg(16)-Gly(17)-Pro-Arg-Val(Acm)-NH{sub 2} (F17), Ala-Asp-Ser-Gly-Glu-Cys(Acm)-Asp(7)-Phe-Leu-Ala-Glu-Val(12)-Gly-Gly-Val-Arg(16) (tF17). Binding of thrombin to peptides F16 and F17, and hence to tF16 and tF17 as a result of the cleavage of the Arg(16)-Gly(17) peptide bond, broadens the proton resonances of residues Asp(7) to Arg(16), suggesting that thrombin interacts specifically with this sequence of residues. Medium- and long-range TRNOE's were observed between the NH proton of Asp(7) and the C{sup {beta}}H protons of Ala(10) and between the ring protons of Phe(8) and the C{sup {gamma}}H protons of Val(12) and Val(15) in complexes of thrombin with both tF16 and tF17. A strong TRNOE, in peptides tF16 and tF17, between the C{sup beta}H protons of Glu(11) and the backbone NH proton of Val(12) was also observed. Thus, the bleeding disorder caused by the single mutation of Gly(12) to Val(12) may be due to the structural alteration of human fibrinogen Rouen identified here.

• OSTI ID: 5546053
• Journal Information: Biochemistry; (USA), Vol. 28:7; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BLOOD COAGULATION
HEREDITARY DISEASES
PEPTIDES
NUCLEAR MAGNETIC RESONANCE
AMINO ACID SEQUENCE
AQUEOUS SOLUTIONS
CATTLE
GLYCINE
HEMORRHAGE
MUTATIONS
OVERHAUSER EFFECT
PROTONS
THROMBIN
VALINE
VASCULAR DISEASES
AMINO ACIDS
ANIMALS
BARYONS
CARBOXYLIC ACIDS
COAGULANTS
DISEASES
DISPERSIONS
DOMESTIC ANIMALS
DRUGS
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
HEMATOLOGIC AGENTS
HEMOSTATICS
HYDROLASES
MAGNETIC RESONANCE
MAMMALS
MIXTURES
MOLECULAR STRUCTURE
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PEPTIDE HYDROLASES
PROTEINS
RESONANCE
RUMINANTS
SERINE PROTEINASES
SOLUTIONS
SYMPTOMS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics