Heteronuclear two-dimensional sup 15 N- and sup 13 C-NMR studies of DNA oligomers and their netropsin complexes using indirect proton detection

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Biopolymers; (USA)

DOI:https://doi.org/10.1002/bip.360310105· OSTI ID:5545622

Ashcroft, J; Live, D H; Patel, D J; Cowburn, D ^[1]

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1. Rockefeller Univ., New York, NY (USA)

Heteronuclear multispin coherence proton-detected two-dimensional nmr spectroscopic experiments were used to obtain information on protonated carbons and nitrogens of the self-complementary d(G-G-T-A-T-A-C-C) and d(G-G-A-A-T-T-C-C) duplexes, with and without the drug netropsin dissolved in aqueous solution. Many correlations of protons coupled to {sup 13}C nuclei on the base and sugar rings of the octanucleotides were detected, allowing the carbon resonances to be assigned based on previous homonuclear proton two-dimensional nmr studies. Imino nitrogen assignments can also be made using the proton assignments from previous one-dimensional nuclear overhauser effect experiments. Imino nitrogen shifts may be useful indicators of changes in local hydrogen-bonding interactions to base-pair edges.

• OSTI ID: 5545622
• Journal Information: Biopolymers; (USA), Vol. 31:1; ISSN 0006-3525
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NUCLEOTIDES
MOLECULAR STRUCTURE
AMINO ACID SEQUENCE
CARBON 13
CHEMICAL SHIFT
DNA
NITROGEN 15
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PROTONS
TRACER TECHNIQUES
BARYONS
CARBON ISOTOPES
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
HADRONS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NITROGEN ISOTOPES
NUCLEI
NUCLEIC ACIDS
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RESONANCE
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques