Benzo(a)pyrene- and aldrin-metabolizing activities in cultured human and rat hepatoma cell lines
Five established hepatoma cell lines, 1 of rat origin and 4 derived from human liver carcinoma, were compared for their capacity to perform metabolic activation of one polycyclic hydrocarbon, benzo(a)pyrene (BP), and one cyclodiene chlorinated insecticide, aldrin. The results of these investigations indicated both species and individual differences among these cell lines. Aldrin was found to be converted into dieldrin much more efficiently by the rat hepatoma cell line than by any human cell lines, whereas 2 human lines displayed the highest BP-metabolizing activity whether measured as the amounts of water-soluble products or estimated by cytotoxic cell-mediated assays. Results also showed that one particular human cell line can replace, advantageously, V79 hamster cells as target in a cell-mediated assay owing to its incapability to metabolize BP and its high sensitivity to BP metabolites.
- Research Organization:
- Laboratoire de Biologie cellulaire et moleculaire de Developpement, Universite Libre de Bruxelles, Belgium
- OSTI ID:
- 5544779
- Journal Information:
- JNCI, J. Natl. Cancer Inst.; (United States), Vol. 71:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ALDRIN
METABOLIC ACTIVATION
BENZOPYRENE
CELL CULTURES
COMPARATIVE EVALUATIONS
HEPATOMAS
MAN
MUTAGEN SCREENING
RATS
TUMOR CELLS
ANIMAL CELLS
ANIMALS
AROMATICS
CHLORINATED AROMATIC HYDROCARBONS
CONDENSED AROMATICS
DISEASES
HALOGENATED AROMATIC HYDROCARBONS
HYDROCARBONS
INSECTICIDES
MAMMALS
NEOPLASMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PESTICIDES
PRIMATES
RODENTS
SCREENING
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)