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Title: Regulation of antifreeze protein messenger RNA and a female-specific messenger RNA in winter flounder: Evidence for temperature and photoperiod effects

Abstract

Northern blot hybridizations showed that mRNA{sub f} is female-specific, and that both mRNAs are liver-specific and expressed at high levels only during the fall and winter in the wild. When warm-acclimated flounder (18C) were acclimated over 2 days to low temperature (4 C) and short photoperiod in July and October, high levels of AF mRNA accumulated within 4-10 days. Low levels were found in fish at high temperature at any time, on long photoperiod after acclimation to low temperature in July, or continuously at 4 C from March until September. A temperature increase in January resulted in a dramatic decrease in the levels of AF mRNA within 5 days. Beta actin mRNA, mRNA{sub f} and most mRNAs translated in a reticulocyte lysate did not change in abundance after temperature increases or decreases. Synthesis of AF mRNA in liver slices was investigated by Northern blot hybridization and hybridization of {sup 32}P-labeled RNA to AF cDNA. Liver slices from cold-acclimated January fish expressed high levels of AF mRNA initially at 4 C and lower levels at 18 C. AF mRNA expression was turned off in tissue from warm-acclimated fish but was progressively induced after long 4 C incubations in vitro in liversmore » from fall fish but not from spring fish. Temperature and photoperiod act either as zeitgebers, which set the phase of an endogenous rhythm, or as more direct signals that stimulate a transient accumulation of AF mRNA.« less

Authors:
Publication Date:
Research Org.:
Johns Hopkins Univ., Baltimore, MD (USA)
OSTI Identifier:
5542692
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; FISHES; TEMPERATURE EFFECTS; MESSENGER-RNA; SPECIFICITY; DNA HYBRIDIZATION; GENE REGULATION; LIVER; PHOSPHORUS 32; SEASONAL VARIATIONS; SEX DEPENDENCE; TRACER TECHNIQUES; TRANSCRIPTION; ANIMALS; AQUATIC ORGANISMS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; GLANDS; HYBRIDIZATION; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RADIOISOTOPES; RNA; VARIATIONS; VERTEBRATES; 560200* - Thermal Effects; 550201 - Biochemistry- Tracer Techniques

Citation Formats

Price, J L. Regulation of antifreeze protein messenger RNA and a female-specific messenger RNA in winter flounder: Evidence for temperature and photoperiod effects. United States: N. p., 1988. Web.
Price, J L. Regulation of antifreeze protein messenger RNA and a female-specific messenger RNA in winter flounder: Evidence for temperature and photoperiod effects. United States.
Price, J L. 1988. "Regulation of antifreeze protein messenger RNA and a female-specific messenger RNA in winter flounder: Evidence for temperature and photoperiod effects". United States.
@article{osti_5542692,
title = {Regulation of antifreeze protein messenger RNA and a female-specific messenger RNA in winter flounder: Evidence for temperature and photoperiod effects},
author = {Price, J L},
abstractNote = {Northern blot hybridizations showed that mRNA{sub f} is female-specific, and that both mRNAs are liver-specific and expressed at high levels only during the fall and winter in the wild. When warm-acclimated flounder (18C) were acclimated over 2 days to low temperature (4 C) and short photoperiod in July and October, high levels of AF mRNA accumulated within 4-10 days. Low levels were found in fish at high temperature at any time, on long photoperiod after acclimation to low temperature in July, or continuously at 4 C from March until September. A temperature increase in January resulted in a dramatic decrease in the levels of AF mRNA within 5 days. Beta actin mRNA, mRNA{sub f} and most mRNAs translated in a reticulocyte lysate did not change in abundance after temperature increases or decreases. Synthesis of AF mRNA in liver slices was investigated by Northern blot hybridization and hybridization of {sup 32}P-labeled RNA to AF cDNA. Liver slices from cold-acclimated January fish expressed high levels of AF mRNA initially at 4 C and lower levels at 18 C. AF mRNA expression was turned off in tissue from warm-acclimated fish but was progressively induced after long 4 C incubations in vitro in livers from fall fish but not from spring fish. Temperature and photoperiod act either as zeitgebers, which set the phase of an endogenous rhythm, or as more direct signals that stimulate a transient accumulation of AF mRNA.},
doi = {},
url = {https://www.osti.gov/biblio/5542692}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}

Thesis/Dissertation:
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