Establishment and characterization of Xenopus oviduct cells in primary culture

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90337-5· OSTI ID:5534038

Marsh, J; Tata, J R ^[1]

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1. National Inst. for Medical Research, London (United Kingdom)

Based on previously established procedure of Xenopus hepatocytes, the authors describe tubular oviduct cells in primary culture which continue to secrete substantial quantities of egg jelly for several days, as can be visualized microscopically. Freshly isolated cells exhibited a culture shock response, from which they recovered by the third day in culture. This recovery was characterized by (a) the diminished synthesis of heat shock proteins hsp 70 and hsp 85, (b) the cessation of the drop in number of estrogen receptor, and (c) the enhanced rate of synthesis of cellular and secreted proteins. The oviduct estrogen receptor had the same characteristics as those in other estrogen target tissues and was present in the same amount as in adult female Xenopus hepatocytes. The successful establishment and characterization of primary cultures of both liver and oviduct cells now fulfill the conditions required for investigating the basis for tissue specificity of regulation by estrogen of Xenopus egg protein gene expression in primary cell culture.

• OSTI ID: 5534038
• Journal Information: Experimental Cell Research; (United States), Vol. 173:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

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AMINO ACIDS
ANIMALS
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HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
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LIPOTROPIC FACTORS
MEMBRANE PROTEINS
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NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
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STEROID HORMONES
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