Simultaneous saccharification and fermentation of cellulose: effect of ethanol on enzymatic saccharification of cellulose. [Trichoderma reesei]
It was confirmed that simultaneous saccharification and fermentation are effective for accelerating enzymatic saccharification of cellulose. In this work, the effects of ethanol on the saccharification of tissue paper by Trichoderma cellulase (Meicelase CEPB) have been investigated. The following results were obtained. 1) Saccharification was inhibited by at least 0.2M ethanol. 2) Less than 4M ethanol did not affect the enzymatic activities of ..beta..-glucosidase and endoglucanase (C/sub x/) at all. The thermal stability of endoglucanase was not also varied by ethanol. 3) It is suggested that ethanol depresses the adsorption of exoglucanase on cellulose. 4) The rate expression of saccharification of cellulose in the presence of ethanol is proposed. 5) The inhibititory effect of ethanol was found to become more significant in the later stages of the reaction than just the initial stage.
- Research Organization:
- Osaka City Univ., Japan
- OSTI ID:
- 5533378
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 27:4
- Country of Publication:
- United States
- Language:
- English
Similar Records
Simultaneous saccharification and fermentation of cellulose to ethanol
Fermentation of crystalline cellulose to ethanol by Klebsiella oxytoca containing chromosomally integrated zymomonas mobilis genes
Related Subjects
CELLULOSE
ENZYMATIC HYDROLYSIS
FERMENTATION
INHIBITION
ETHANOL
BIOSYNTHESIS
ENZYME ACTIVITY
EXPERIMENTAL DATA
FUNGI
GLYCOSYL HYDROLASES
ALCOHOLS
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
DATA
DECOMPOSITION
ENZYMES
HYDROLASES
HYDROLYSIS
HYDROXY COMPOUNDS
INFORMATION
LYSIS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
SYNTHESIS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)