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Title: Structure and assembly of the flagellar hook-basal body complex of Salmonella typhimurium

Abstract

The hook-basal body (HBB) complex is a multi-component structure which comprises a significant part of the bacterial flagellar motor. Electrophoretic mobility shifts of HBB complex component proteins from four non-flagellate mutants have enabled the author to assign each protein as being the product of the gene defective in each of the respective strains. The author has purified and characterized HBB complexes lacking either the L ring or both the P and L rings, and concluded that the 27-kDa basal-body protein is the major component of the L ring, and that the 38-kDa basal-body protein is the major component of the P ring. He has sequenced the genes encoding the subunit proteins of the M, P, and L rings of the basal body, and have examined both the gene and deduced amino acid sequences for clues regarding the regulation of these genes and the structure of their products. By quantitating the amount of {sup 35}S incorporated into the component protein vivo and correcting for the amount of contained in each protein (as deduced from gene sequencing data), he has determined the relative stoichiometries of most of the known component proteins of the HBB complex. He has developed a protocol for differentialmore » {sup 35}S-radiolabeling of HBB complexes in vivo and used it to examine the HBB complex assembly process. He has identified proteins required for M-ring assembly or stabilization and for the possible initiation of rod assembly. The rod is not stable until the P ring is assembled onto it. The monomers of the P and L rings are exported independent of flagellar assembly. These radiolabeling experiments have also enabled me to identify several new component proteins of the HBB complex.« less

Authors:
Publication Date:
Research Org.:
Yale Univ., New Haven, CT (USA)
OSTI Identifier:
5526781
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEINS; MOLECULAR STRUCTURE; SALMONELLA TYPHIMURIUM; MOLECULAR BIOLOGY; AMINO ACID SEQUENCE; CHEMICAL COMPOSITION; ELECTROPHORESIS; GENE REGULATION; GENES; SULFUR 35; TRACER TECHNIQUES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; EVEN-ODD NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; SALMONELLA; SULFUR ISOTOPES 550201* -- Biochemistry-- Tracer Techniques; 550701 -- Microbiology-- Tracer Techniques

Citation Formats

Jones, C.J. Structure and assembly of the flagellar hook-basal body complex of Salmonella typhimurium. United States: N. p., 1989. Web.
Jones, C.J. Structure and assembly of the flagellar hook-basal body complex of Salmonella typhimurium. United States.
Jones, C.J. 1989. "Structure and assembly of the flagellar hook-basal body complex of Salmonella typhimurium". United States. doi:.
@article{osti_5526781,
title = {Structure and assembly of the flagellar hook-basal body complex of Salmonella typhimurium},
author = {Jones, C.J.},
abstractNote = {The hook-basal body (HBB) complex is a multi-component structure which comprises a significant part of the bacterial flagellar motor. Electrophoretic mobility shifts of HBB complex component proteins from four non-flagellate mutants have enabled the author to assign each protein as being the product of the gene defective in each of the respective strains. The author has purified and characterized HBB complexes lacking either the L ring or both the P and L rings, and concluded that the 27-kDa basal-body protein is the major component of the L ring, and that the 38-kDa basal-body protein is the major component of the P ring. He has sequenced the genes encoding the subunit proteins of the M, P, and L rings of the basal body, and have examined both the gene and deduced amino acid sequences for clues regarding the regulation of these genes and the structure of their products. By quantitating the amount of {sup 35}S incorporated into the component protein vivo and correcting for the amount of contained in each protein (as deduced from gene sequencing data), he has determined the relative stoichiometries of most of the known component proteins of the HBB complex. He has developed a protocol for differential {sup 35}S-radiolabeling of HBB complexes in vivo and used it to examine the HBB complex assembly process. He has identified proteins required for M-ring assembly or stabilization and for the possible initiation of rod assembly. The rod is not stable until the P ring is assembled onto it. The monomers of the P and L rings are exported independent of flagellar assembly. These radiolabeling experiments have also enabled me to identify several new component proteins of the HBB complex.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = 1989,
month = 1
}

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  • The flagellar root system of Entosiphon sulcatum (Dujardin) Stein (Euglenophyceae) is described and compared with kinetoplastid and other euglenoid systems. An asymmetric pattern of three microtubular roots, one between the two flagellar basal bodies and one on either side (here called the intermediate, dorsal, and ventral roots), is consistent within the euglenoid flagellates studied thus far. The dorsal root is associated with the basal body of the anterior flagellum (F1) and lies on the left dorsal side of the basal body complex. Originating between the two flagellar basal bodies, and associated with the basal body of the trailing flagellum (F2),more » the intermediate root is morphologically distinguished by fibrils interconnecting the individual microtubules to one another and to the overlying reservoir membrane. The intermediate root is often borne on a ridge projecting into the reservoir. The ventral root originates near the F2 basal body and lies on the right ventral side of the cell. Fibrillar connections link the membrane of F2 with the reservoir membrane at the reservoir-canal transition level. A large cross-banded fiber joins the two flagellar basal bodies, and a series of smaller striated fibers links the anterior accessory and flagellar basal bodies. Large nonstriated fibers extend from the basal body complex posteriorly into the cytoplasm.« less
  • Drawings are given for an 84 mm body-transition assembly. Details include extruding penetrator, afterbody, gas nozzles, tubes, insulators, thermal shield, transition block and related parts, seal, electrodes, springs, sleeves, heaters, and formers. (PMA)
  • This dissertation concerns the development and application of new techniques for electronic structure calculations motivated by dimensional scaling (analytic continuation in the spatial dimensionality D followed by finitizing scalings). One desirable feature of dimensional scaling as applied to electronic structure problems is that it gives rise to two distinct singular (and hence simplifying) limits, namely D [yields] 1 and D [yields] [infinity]. A scaling procedure which is finitizing and uniform for 1 [le] D [le] [infinity] is presented. Dimensional limit results obtained with this scaling can be used to obtain quite accurate approximations to D = 3 eigenvalues. The proceduremore » is demonstrated for H[sub 2][sup +] and for H[sub 2] in the Hartree approximation. For the latter problem both the D [yields] 1 and D [yields] [infinity] solutions are obtained for the first time. Another advantage of the dimensional scaling approach is its usefulness for studying correlation effects. This is demonstrated for the model many-body problem of N mutually gravitating bosons. The exact and Hartree D [yields] [infinity] solutions are derived (both in closed form), and combined with literature results for the exact and Hartree D [yields] 1 solutions (also both in closed form) to obtain approximate D = 3 solutions. For comparison, the Hartree D = 3 solution is also solved numerically. The dimensionally generalized hamiltonian used for this problem is obtained in a quite general form which should also be useful for other problems. A third benefit of dimensional scaling is that it provides conceptually simple models of electronic structure. The D [yields] [infinity] limit yields classical structures which are useful but by themselves are at best qualitatively correct. A procedure for generating classical representations which incorporates finite-D effects is presented.« less