Identification and characterization of Vibrio cholerae surface proteins by radioiodination
Whole cells and isolated outer membrane from Vibrio cholerae (Classical, Inaba) were radiolabeled with Iodogen or Iodo-beads as catalyst. Radiolabeling of whole cells was shown to be surface specific by sodium dodecyl sulfate-urea polyacrylamide gel electrophoresis of whole cells and cell fractions. Surface-labeled whole cells regularly showed 16 distinguishable protein species, of which nine were found in radiolabeled outer membrane preparations obtained by a lithium chloride- lithium acetate procedure. Eight of these proteins were found in outer membranes prepared by sucrose density gradient centrifugation and Triton X-100 extraction of radiolabeled whole cells. The mobility of several proteins was shown to be affected by temperature, and the major protein species exposed on the cell surface was shown to consist of at least two different peptides.
- Research Organization:
- Univ. of Missouri, Columbia
- OSTI ID:
- 5520732
- Journal Information:
- Infect. Immun.; (United States), Vol. 48:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
CHEMICAL COMPOSITION
BACTERIA
CELL MEMBRANES
ELECTROPHORESIS
IODINE ISOTOPES
LABELLED COMPOUNDS
TEMPERATURE EFFECTS
TRACER TECHNIQUES
CELL CONSTITUENTS
ISOTOPE APPLICATIONS
ISOTOPES
MEMBRANES
MICROORGANISMS
ORGANIC COMPOUNDS
550201* - Biochemistry- Tracer Techniques