Effect of various eicosanoid products of arachidonic acid on the acyl CoA: Cholesterol acyl transferase activity in three different mammalian cell lines
Acylcoenzyme A:cholesterol acyltransferase (ACAT) catalyzes cholesterol ester synthesis intracellularly and has been implicated in the development of atherosclerosis. An in vitro assay has been adapted for determining ACAT activity from rat FU5AH hepatoma, Chinese hamster ovary (CHO) and rat thoracic aortic smooth muscle (RSM) cells. Formation of {sup 14}C-labelled cholesteryl oleate at 0 to 60 min {plus minus} cholesterol was determined; in the presence of exogenous cholesterol, ACAT activity was approximately linear and surpassed the plateau observed in ACAT activity without cholesterol. Increasing exogenous cholesterol concentration, the amount of oleoyl CoA or the amount of microsomal protein produced a corresponding increase in ACAT activity, while ester formation was slightly increased by decreasing the ratio of Triton WR-1339 to cholesterol. Both the thromboxane A{sub 2} (TxA{sub 2}) mimic, U-44069, and the inflammatory lipoxygenase product, LTB{sub 4}, decreased optimal in vitro microsomal ACAT activity from RSM, but not form FU5AH, while CHO ACAT activity was suppressed by LTB{sub r} only. PGI{sub 2}, PGE{sub 2} and PGF{sub 2{alpha}} had minimal effects for each cell type.
- Research Organization:
- Drexel Univ., Philadelphia, PA (USA)
- OSTI ID:
- 5516960
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARTERIOSCLEROSIS
PATHOGENESIS
CHOLESTEROL
BIOSYNTHESIS
EICOSANOIC ACID
BIOCHEMICAL REACTION KINETICS
TRANSFERASES
ENZYME ACTIVITY
ARACHIDONIC ACID
CARBON 14 COMPOUNDS
CHO CELLS
ESTERS
HEPATOMAS
MUSCLES
RADIOASSAY
RATS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
DISEASES
ENZYMES
HYDROXY COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
NEOPLASMS
ORGANIC ACIDS
ORGANIC COMPOUNDS
REACTION KINETICS
RODENTS
STEROIDS
STEROLS
SYNTHESIS
VASCULAR DISEASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques