Platelet-derived growth factor receptor: Studies examining synthesis, phosphorylation and degradation of the receptor using an anti-receptor monoclonal antibody
A monoclonal antibody, designated PR7212 (IgG1), has been developed with specifically recognizes a cell-surface receptor for platelet-derived growth factor (PDGF). The antibody recognizes an extracellular epitope of the receptor, demonstrated by its ability to bind to intact cells. Using this antibody I have detected three forms of the receptor of 180, 164, and 130 kDa. All three forms were detected by Western blot analysis of human dermal fiberblasts. Immunoprecipitates of {sup 32}P-labeled membrane extracts of human dermal fibroblasts demonstrate that phosphorylation of all three forms of the receptor is stimulated by PDGF. In addition, several smaller molecules were detected, ranging in size from 113 to 49 kDa, which are also phosphorylated in response to PDGF addition. These smaller molecules may be either PDGF receptor kinase substrates or partially degraded receptor. Only the 180 and the 164 kDa forms of the receptor are detectable from immunoprecipitates of soluble extracts of {sup 35}S-metabolically labeled cells. Pulse-chase experiments demonstrate that the 164 kDa form is a precursor of the 180 kDa molecule.
- Research Organization:
- Washington Univ., Seattle, WA (USA)
- OSTI ID:
- 5516601
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GROWTH FACTORS
RECEPTORS
MONOCLONAL ANTIBODIES
SPECIFICITY
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BLOOD PLATELETS
CELL MEMBRANES
FIBROBLASTS
MAN
METABOLISM
PHOSPHORUS 32
PHOSPHOTRANSFERASES
SULFUR 35
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques