Active glucose transport and proton pumping in tonoplast membrane of Zea mays L. coleoptiles are inhibited by anti-H/sup +/-ATPase antibodies

Journal Article · Tue Dec 01 00:00:00 EST 1987 · Plant Physiol.; (United States)

OSTI ID:5515820

Rausch, T; Butcher, D N; Taiz, L

A tonoplast enriched fraction was obtained from Zea mays L. coleoptiles by isopycnic centrifugation of microsomal membranes in a sucrose step gradient. At the 18/26% interface chloride-stimulated and nitrate-inhibited proton pumping activity coincided with a Mg/sup 2 +/-ATP dependent accumulation of 3-O-methyl-D-glucose (OMG) as determined by a membrane filtration technique using /sup 14/C-labeled substrate. OMG transport showed an apparently saturable component with a K/sub m/ of 110 micromolar, and was completely inhibited by 10 micromolar carbonyl cyanide m-chlorophenylhydrazone. Polyclonal antibodies against solubilized native tonoplast H/sup +/-ATPase and its 62 and 72 kilodalton subunits were assayed for their ability to inhibit proton pumping and OMG accumulation. Antibodies against both the native enzyme and the putative catalytic subunit strongly inhibited proton pumping and OMG transport whereas antibodies against the 62 kilodalton subunit had only a slight effect on both processes.

• Research Organization: Johann Wolfgang Goethe Universitaet, Frankfurt, Germany, F.R.
• OSTI ID: 5515820
• Journal Information: Plant Physiol.; (United States), Vol. 85:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
ATP-ASE
GLUCOSE
MEMBRANE TRANSPORT
CARBON 14 COMPOUNDS
INHIBITION
MAIZE
MICROSOMES
PHOTOSYNTHETIC MEMBRANES
PROTONS
TRACER TECHNIQUES
ACID ANHYDRASES
ALDEHYDES
BARYONS
CARBOHYDRATES
CELL CONSTITUENTS
CEREALS
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
GRASS
HADRONS
HEXOSES
HYDROLASES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANES
MONOSACCHARIDES
NUCLEONS
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHOHYDROLASES
PLANTS
REACTION KINETICS
SACCHARIDES
550201* - Biochemistry- Tracer Techniques