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Title: Two-dimensional sup 1 H NMR studies of synthetic immobile Holliday junctions

Abstract

Two 32 base pair, four-arm immobile Holliday junctions have been prepared and studied by two-dimensional {sup 1}H nuclear magnetic resonance (NMR) spectroscopy. Two-quantum spectroscopy provides scalar (through bond) correlations for the 1{prime}H, 2{prime}H, and 2{double prime}H resonances of the deoxyribose sugar rings and the nonlabile cytosine and thymine base protons. Assignments in the deoxyribose sugars are extended to the 3{prime}H resonances principally from relayed connectivities in total correlation spectra. Severe overlap of resonances in the standard regions of two-dimensional nuclear Overhauser enhancement (NOE) spectra necessitated the use of a unique approach for obtaining sequence-specific assignments of duplex DNA, wherein all possible NOE connectivities in the spectra are analyzed. These studies of 64-residue structures represent a substantial step forward with respect to the size of oligonucleotide for which virtually complete assignments are obtained. The assignments form the critical background for the detailed analysis of Holliday junction structure and dynamics that is required to address key issues in understanding the role of Holliday junctions in genetic recombination and repair.

Authors:
; ; ;  [1]
  1. Scripps Clinic and Research Foundation, La Jolla, CA (USA)
Publication Date:
OSTI Identifier:
5510562
Resource Type:
Journal Article
Journal Name:
Biochemistry; (United States)
Additional Journal Information:
Journal Volume: 30:3; Journal ID: ISSN 0006-2960
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA; MOLECULAR BIOLOGY; OLIGONUCLEOTIDES; NUCLEAR MAGNETIC RESONANCE; CYTOSINE; DNA REPAIR; GENE RECOMBINATION; PROTONS; THYMINE; AMINES; AZINES; BARYONS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; ELEMENTARY PARTICLES; FERMIONS; HADRONS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MAGNETIC RESONANCE; NUCLEIC ACIDS; NUCLEONS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PYRIMIDINES; RECOVERY; REPAIR; RESONANCE; URACILS; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Chen, Shiowmeei, Heffron, F, Leupin, W, and Chazin, W J. Two-dimensional sup 1 H NMR studies of synthetic immobile Holliday junctions. United States: N. p., 1991. Web. doi:10.1021/bi00217a028.
Chen, Shiowmeei, Heffron, F, Leupin, W, & Chazin, W J. Two-dimensional sup 1 H NMR studies of synthetic immobile Holliday junctions. United States. doi:10.1021/bi00217a028.
Chen, Shiowmeei, Heffron, F, Leupin, W, and Chazin, W J. Tue . "Two-dimensional sup 1 H NMR studies of synthetic immobile Holliday junctions". United States. doi:10.1021/bi00217a028.
@article{osti_5510562,
title = {Two-dimensional sup 1 H NMR studies of synthetic immobile Holliday junctions},
author = {Chen, Shiowmeei and Heffron, F and Leupin, W and Chazin, W J},
abstractNote = {Two 32 base pair, four-arm immobile Holliday junctions have been prepared and studied by two-dimensional {sup 1}H nuclear magnetic resonance (NMR) spectroscopy. Two-quantum spectroscopy provides scalar (through bond) correlations for the 1{prime}H, 2{prime}H, and 2{double prime}H resonances of the deoxyribose sugar rings and the nonlabile cytosine and thymine base protons. Assignments in the deoxyribose sugars are extended to the 3{prime}H resonances principally from relayed connectivities in total correlation spectra. Severe overlap of resonances in the standard regions of two-dimensional nuclear Overhauser enhancement (NOE) spectra necessitated the use of a unique approach for obtaining sequence-specific assignments of duplex DNA, wherein all possible NOE connectivities in the spectra are analyzed. These studies of 64-residue structures represent a substantial step forward with respect to the size of oligonucleotide for which virtually complete assignments are obtained. The assignments form the critical background for the detailed analysis of Holliday junction structure and dynamics that is required to address key issues in understanding the role of Holliday junctions in genetic recombination and repair.},
doi = {10.1021/bi00217a028},
journal = {Biochemistry; (United States)},
issn = {0006-2960},
number = ,
volume = 30:3,
place = {United States},
year = {1991},
month = {1}
}