Observations on B cells of progeny and mothers exposed to benzo(a)pyrene at midgestation
Defects exist in the T cell population of benzo(a)pyrene (BP) exposed mothers and in their immunodeficient progeny, but information is needed on the behavior of their B cells. The authors are analyzing the ability of splenic B cells from BP-exposed progeny and mothers to produce ..gamma..1, ..gamma..2b, and ..gamma..3 in vitro in the presence of LPS and T cells from control- or BP-exposed mice, or supernatants (containing IL-2) from cultures of allogeneic mouse spleen cells and Con A stimulated mouse or rat cells. Combinations of T and B cells from normal, control or BP-exposed mice were cultured for 5 days and secreted isotypes assayed by ELISA using monoclonal rabbit ..cap alpha..-mouse Ig. Preliminary results are: a) a 3- to 4-fold reduction in production of ..gamma..3 by B cells from BP-exposed progeny but no change in ..gamma..1 or ..gamma..2b, b) B cells mixed with control or BP T cells showed reduction in all 3 isotypes. In cultures of T and B cells from BP-injected mothers the 3 isotypes were enhanced. In the presence of IL-2, ..gamma..1 and ..gamma..3 were diminished in B cells from BP progeny, while the mothers showed enhancement of the 3 Ig's. Thus, BP inhibits IgG production by progeny B cells in the presence or absence of growth or differentiation factors, but enhances Ig production by the mother. The data suggest disruptions in T and B cell interactions, a condition which may have severe immunologic consequences including the response against tumors.
- Research Organization:
- Moorehouse School of Medicine, Atlanta, GA
- OSTI ID:
- 5499317
- Report Number(s):
- CONF-8604222-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BENZOPYRENE
BIOLOGICAL EFFECTS
IMMUNOGLOBULINS
INHIBITION
CELL CULTURES
LYMPHOCYTES
MICE
MONOCLONAL ANTIBODIES
PREGNANCY
PROGENY
RATS
SPLEEN CELLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
GLOBULINS
HYDROCARBONS
LEUKOCYTES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)