In vitro binding of selenium by rat liver mitochondrial selenium-binding protein
Last year the authors reported that upon freezing and thawing mitochondria from rats injected with (/sup 75/Se)Na/sub 2/SeO/sub 3/ (/sup 75/Se-selenite), a /sup 75/Se-binding protein (SeBP) was released. They have studied further in vitro labelling of SeBP. This matrix protein was labelled in vitro when lysed mitochondria (containing non-matrix material) were incubated with /sup 75/Se-selenite but not when matrix material alone was incubated with /sup 75/Se-selenite. Thus, there are one or more promoters of in vitro SeBP labelling in the non-matrix fraction. SeBP was also labelled in vitro when /sup 75/Se-selenite was added to matrix alone and dialyzed. Dialysis tubing, and not the dialysis process, promoted labelling by affecting SeBP and not by affecting /sup 75/Se-selenite. Labelling did not occur when matrix alone and /sup 75/Se-selenite were incubated (not dialyzed) in a glass test tube but did occur in a polystyrene test tube. They hypothesize that non-covalent interactions occur between SeBP and dialysis tubing or polystyrene that expose Se binding sites on the protein. A similar mechanism involving mitochondrial non-matrix material may function in vivo. Non-denaturing disc gel electrophoresis of partially purified SeBP labelled in vivo or in vitro suggested that the same protein was labelled in both conditions. Using in vitro binding techniques, SeBP was also found in sheep liver mitochondrial matrix. This supports the theory that SeBP is important in Se metabolism.
- Research Organization:
- Univ. of Wisconsin, Madison
- OSTI ID:
- 5498352
- Report Number(s):
- CONF-8604222-; TRN: 86-026828
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
PROTEINS
LABELLING
SELENIUM
BIOCHEMICAL REACTION KINETICS
ELECTROPHORESIS
IN VITRO
LIVER
MITOCHONDRIA
RATS
RECEPTORS
SELENIUM 75
TRACER TECHNIQUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELECTRON CAPTURE RADIOISOTOPES
ELEMENTS
EVEN-ODD NUCLEI
GLANDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SELENIUM ISOTOPES
SEMIMETALS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550201 - Biochemistry- Tracer Techniques