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Title: Influence of dietary iron level and form on biochemical, hematological, and immunological changes in copper deficient rats

Abstract

Weanling male Lewis rats (N = 10/group) were fed ad-libitum for 42 days diets based on AIN standards containing 21% casein, 5% safflower oil, deficient (0.6 ..mu..g/g) or adequate (5.6 ..mu..g/g) levels of Cu, and adequate (50 ..mu..g/g) or high (300 ..mu..g/g) levels of Fe/sup +2/ or Fe/sup +3/. Cu-deficient rats, regardless of Fe level or form, exhibited depressed (p < 0.05) serum Cu, Fe and ceruloplasmin activity, and hemoglobin levels; and elevated (p < 0.05) unsaturated serum Fe binding capacity. Except for high Fe/sup +3/ fed rats, Cu-deficient rats showed decreased hematocrits. Decreased proliferation was exhibited by concanavalin-A (Con-A) stimulated spleen lymphoid cells (SLC) of Cu-deficient rats fed adequate dietary Fe, but not by SLC of Cu-deficient rats fed high dietary Fe. High Fe fed rats exhibited reduced proliferation and increased variability in proliferation by Con-A stimulated SLC, which apparently caused a lack of difference in proliferation by SLC of Cu-deficient and Cu-adequate rats fed high Fe. Thus, high dietary Fe did not correct biochemical and hematological parameters in Cu-deficient rats, but because of lowered proliferation and increased variability of SLC proliferation, high dietary Fe did alleviate suppressed Con-A stimulated SLC proliferation in Cu-deficiency.

Authors:
; ;
Publication Date:
Research Org.:
Dept. of Agriculture, Grand Forks, ND
OSTI Identifier:
5498199
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; COPPER; NUTRITIONAL DEFICIENCY; IRON; BIOLOGICAL EFFECTS; SPLEEN CELLS; CELL PROLIFERATION; BLOOD COUNT; CONCANAVALIN; DIET; HEMOGLOBIN; RATS; AGGLUTININS; ANIMAL CELLS; ANIMALS; ANTIBODIES; CARBOXYLIC ACIDS; ELEMENTS; GLOBIN; HEMAGGLUTININS; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; LECTINS; MAMMALS; METALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PIGMENTS; PORPHYRINS; PROTEINS; RODENTS; SOMATIC CELLS; TRANSITION ELEMENTS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Leu, H, Gallaher, D D, and Kramer, T R. Influence of dietary iron level and form on biochemical, hematological, and immunological changes in copper deficient rats. United States: N. p., 1986. Web.
Leu, H, Gallaher, D D, & Kramer, T R. Influence of dietary iron level and form on biochemical, hematological, and immunological changes in copper deficient rats. United States.
Leu, H, Gallaher, D D, and Kramer, T R. Sat . "Influence of dietary iron level and form on biochemical, hematological, and immunological changes in copper deficient rats". United States.
@article{osti_5498199,
title = {Influence of dietary iron level and form on biochemical, hematological, and immunological changes in copper deficient rats},
author = {Leu, H and Gallaher, D D and Kramer, T R},
abstractNote = {Weanling male Lewis rats (N = 10/group) were fed ad-libitum for 42 days diets based on AIN standards containing 21% casein, 5% safflower oil, deficient (0.6 ..mu..g/g) or adequate (5.6 ..mu..g/g) levels of Cu, and adequate (50 ..mu..g/g) or high (300 ..mu..g/g) levels of Fe/sup +2/ or Fe/sup +3/. Cu-deficient rats, regardless of Fe level or form, exhibited depressed (p < 0.05) serum Cu, Fe and ceruloplasmin activity, and hemoglobin levels; and elevated (p < 0.05) unsaturated serum Fe binding capacity. Except for high Fe/sup +3/ fed rats, Cu-deficient rats showed decreased hematocrits. Decreased proliferation was exhibited by concanavalin-A (Con-A) stimulated spleen lymphoid cells (SLC) of Cu-deficient rats fed adequate dietary Fe, but not by SLC of Cu-deficient rats fed high dietary Fe. High Fe fed rats exhibited reduced proliferation and increased variability in proliferation by Con-A stimulated SLC, which apparently caused a lack of difference in proliferation by SLC of Cu-deficient and Cu-adequate rats fed high Fe. Thus, high dietary Fe did not correct biochemical and hematological parameters in Cu-deficient rats, but because of lowered proliferation and increased variability of SLC proliferation, high dietary Fe did alleviate suppressed Con-A stimulated SLC proliferation in Cu-deficiency.},
doi = {},
url = {https://www.osti.gov/biblio/5498199}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {1986},
month = {3}
}

Conference:
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