Radiochemical study of irreversible adsorption of proteins on reversed-phase chromatographic packing materials

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(86)90104-1· OSTI ID:5498125

Sadek, P C; Carr, P W; Bowers, L D; Haddad, L C

A radiochemical study of the irreversible adsorption of proteins on commercial reversed-phase HPLC packing materials is reported. The conditions of study are similar to those used in HPLC separation of protein. The effects of the amounts and contact time of two proteins, ovalbumin and cytochrome c, are reported. Additional results include the effect of column pretreatment with protein, silanophilic, mobile-phase blocking agent, and type of packing material on the extent of irreversible adsorption. The loss process is shown to be at least biphasic and the mechanisms of loss distinct for different proteins.

• Research Organization: Univ. of Minnesota, Minneapolis
• OSTI ID: 5498125
• Journal Information: Anal. Biochem.; (United States), Vol. 153:2, Issue 2
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
LIQUID COLUMN CHROMATOGRAPHY
PACKINGS
PROTEINS
ADSORPTION
COLUMN PACKING
CYTOCHROMES
EXTRACTION COLUMNS
LOSSES
OVALBUMIN
RADIOCHEMICAL ANALYSIS
CARBOHYDRATES
CHEMICAL ANALYSIS
CHROMATOGRAPHY
EXTRACTION APPARATUSES
GLUCOPROTEINS
ORGANIC COMPOUNDS
PIGMENTS
QUANTITATIVE CHEMICAL ANALYSIS
SACCHARIDES
SEPARATION EQUIPMENT
SEPARATION PROCESSES
SORPTION
400103* - Radiometric & Radiochemical Procedures- (-1987)
550201 - Biochemistry- Tracer Techniques