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Title: Heavy metal-induced gene expression in fish and fish cell lines

Abstract

Two isoforms of metallothionein (MT) have been isolated from rainbow trout livers following CdCl/sub 2/ injections. These MTs have been identified by standard procedures and appear to be similar to mammalian MTs. Total RNA from such induced livers was shown to contain high levels of MT-mRNA activity when translated in cell free systems. This activity was demonstrated to be in the 8 to 10S region of a sucrose gradient. The RNA fractions also showed homology to a mouse MT-I cDNA probe. The exposure of rainbow trout hepatoma (RTH) cells to various concentrations of CdCl/sub 2/ and ZnCl/sub 2/ induced the expression of MT and MT-mRNA. Exposure of Chinook salmon embryonic (CHSE) cells to these metals, however, did not result in MT synthesis, suggesting that the MT genes have not become committed to transcription. Instead, an unknown low molecular weight (MW = 14 kDa) protein was induced. This metal-inducible protein (MIP) was capable of binding /sup 109/Cd and was stable to heating, while the binding of the metal to this protein was not. These characteristics have been reported for a protein induced in rainbow trout liver following environmental exposure to cadmium.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Calgary, Alberta
OSTI Identifier:
5497952
Resource Type:
Journal Article
Journal Name:
Environ. Health Perspect.; (United States)
Additional Journal Information:
Journal Volume: 65
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; CADMIUM CHLORIDES; BIOLOGICAL EFFECTS; METALLOPROTEINS; ZINC CHLORIDES; ANIMAL CELLS; FISHES; LIVER; MESSENGER-RNA; METALLOTHIONEIN; RECOMBINANT DNA; TROUT; TUMOR CELLS; ANIMALS; AQUATIC ORGANISMS; BODY; CADMIUM COMPOUNDS; CADMIUM HALIDES; CHLORIDES; CHLORINE COMPOUNDS; DIGESTIVE SYSTEM; DNA; GLANDS; HALIDES; HALOGEN COMPOUNDS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RNA; VERTEBRATES; ZINC COMPOUNDS; ZINC HALIDES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987); 550601 - Medicine- Unsealed Radionuclides in Diagnostics; 550201 - Biochemistry- Tracer Techniques

Citation Formats

Price-Haughey, J, Bonham, K, and Gedamu, L. Heavy metal-induced gene expression in fish and fish cell lines. United States: N. p., 1986. Web. doi:10.2307/3430174.
Price-Haughey, J, Bonham, K, & Gedamu, L. Heavy metal-induced gene expression in fish and fish cell lines. United States. https://doi.org/10.2307/3430174
Price-Haughey, J, Bonham, K, and Gedamu, L. 1986. "Heavy metal-induced gene expression in fish and fish cell lines". United States. https://doi.org/10.2307/3430174.
@article{osti_5497952,
title = {Heavy metal-induced gene expression in fish and fish cell lines},
author = {Price-Haughey, J and Bonham, K and Gedamu, L},
abstractNote = {Two isoforms of metallothionein (MT) have been isolated from rainbow trout livers following CdCl/sub 2/ injections. These MTs have been identified by standard procedures and appear to be similar to mammalian MTs. Total RNA from such induced livers was shown to contain high levels of MT-mRNA activity when translated in cell free systems. This activity was demonstrated to be in the 8 to 10S region of a sucrose gradient. The RNA fractions also showed homology to a mouse MT-I cDNA probe. The exposure of rainbow trout hepatoma (RTH) cells to various concentrations of CdCl/sub 2/ and ZnCl/sub 2/ induced the expression of MT and MT-mRNA. Exposure of Chinook salmon embryonic (CHSE) cells to these metals, however, did not result in MT synthesis, suggesting that the MT genes have not become committed to transcription. Instead, an unknown low molecular weight (MW = 14 kDa) protein was induced. This metal-inducible protein (MIP) was capable of binding /sup 109/Cd and was stable to heating, while the binding of the metal to this protein was not. These characteristics have been reported for a protein induced in rainbow trout liver following environmental exposure to cadmium.},
doi = {10.2307/3430174},
url = {https://www.osti.gov/biblio/5497952}, journal = {Environ. Health Perspect.; (United States)},
number = ,
volume = 65,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1986},
month = {Sat Mar 01 00:00:00 EST 1986}
}