Identification and purification of truncated insulin-like growth factor I from porcine uterus. Evidence for high biological potency

Journal Article · Tue Mar 21 00:00:00 EST 1989 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00432a052· OSTI ID:5473143

Ogasawara, M; Karey, K P; Marquardt, H; Sirbasku, D A ^[1]

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1. Univ. of Texas Medical School, Houston (USA)

The authors report the completion of the purification of uterine-derived growth factors (UDGF) described previously by this laboratory. During isolation, the mitogenic activity was monitored by using the human MCF-7 breast cancer cells in serum-free Ham's F12 and Dulbecco's modified Eagle's medium (1:1, v/v) containing 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, 200 {mu}g/mL bovine serum albumin, and 10 {mu}g/mL human transferrin. This medium sustained growth for several days in response to a single addition of growth factor. The mitogens were shown by protein microsequencing to be DES 1 {yields} 3 to DES 1 {yields} 6 forms of insulin-like growth factor I (truncated IGF-I). An M{sub r} estimated by {sup 125}I labeling, urea-sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and autoradiography was consistent with a DES 1 {yields} 3(4) N{sup {alpha}} truncation. Immunoadsorption and radioimmunoassay confirmed immunological properties equivalent to IGF-I. Radioreceptor assays showed truncated IGF-I was functionally equivalent to recombinant IGF-I. They conclude that the major acid-stable low-M{sub r} mitogenic activities isolated from uterus are very potent forms of truncated IGF-I capable of stimulating growth of epithelial and mesenchymal cells.

• OSTI ID: 5473143
• Journal Information: Biochemistry; (USA), Vol. 28:6; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MITOGENS
MOLECULAR STRUCTURE
TUMOR CELLS
RADIORECEPTOR ASSAY
AUTORADIOGRAPHY
ELECTROPHORESIS
GROWTH FACTORS
INSULIN
IODINE 125
MAMMARY GLANDS
PURIFICATION
SWINE
WOMEN
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
FEMALES
GLANDS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques