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Title: Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro

Abstract

To examine the effects of in vivo eicosapentaenoic acid (EPA) on natural killer (NK) cell activity, C3H/He mice each received a single intraperitoneal bolus of an emulsion of trieicosapentaenoyl-glycerol (EPA-TG). Spleen cells were tested for NK activity using /sup 51/Chromium-release assays against YAC-1 target cells. Forty eight hours after injection, NK activity was inhibited in a dose-dependent manner. EPA-TG emulsion also inhibited the NK activity of NK-enriched effector cells. Decreased cytotoxicity was first noted 24 hr after injection; it resumed the baseline by 7 days. The addition of EPA-TG emulsion to a cytotoxicity assay system resulted in moderate depression of NK activity. These results demonstrate that EPA has significant immunomodulatory effects on NK activity.

Authors:
; ; ;
Publication Date:
Research Org.:
Toyama Medical and Pharmaceutical Univ., Japan
OSTI Identifier:
5471684
Resource Type:
Journal Article
Journal Name:
Biochem. Biophys. Res. Commun.; (United States)
Additional Journal Information:
Journal Volume: 150:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EICOSANOIC ACID; BIOCHEMICAL REACTION KINETICS; LYMPHOCYTES; CELL PROLIFERATION; CELL KILLING; CHROMIUM 51; DOSE-RESPONSE RELATIONSHIPS; EMULSIONS; IN VITRO; IN VIVO; INHIBITION; MICE; MONOCYTES; SPLEEN CELLS; TRACER TECHNIQUES; TRIGLYCERIDES; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CHROMIUM ISOTOPES; COLLOIDS; CONNECTIVE TISSUE CELLS; DISPERSIONS; ELECTRON CAPTURE RADIOISOTOPES; ESTERS; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LEUKOCYTES; LIPIDS; MAMMALS; MATERIALS; MONOCARBOXYLIC ACIDS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Yamashita, N, Sugiyama, E, Hamazaki, T, and Yano, S. Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro. United States: N. p., 1988. Web. doi:10.1016/0006-291X(88)90548-7.
Yamashita, N, Sugiyama, E, Hamazaki, T, & Yano, S. Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro. United States. https://doi.org/10.1016/0006-291X(88)90548-7
Yamashita, N, Sugiyama, E, Hamazaki, T, and Yano, S. 1988. "Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro". United States. https://doi.org/10.1016/0006-291X(88)90548-7.
@article{osti_5471684,
title = {Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro},
author = {Yamashita, N and Sugiyama, E and Hamazaki, T and Yano, S},
abstractNote = {To examine the effects of in vivo eicosapentaenoic acid (EPA) on natural killer (NK) cell activity, C3H/He mice each received a single intraperitoneal bolus of an emulsion of trieicosapentaenoyl-glycerol (EPA-TG). Spleen cells were tested for NK activity using /sup 51/Chromium-release assays against YAC-1 target cells. Forty eight hours after injection, NK activity was inhibited in a dose-dependent manner. EPA-TG emulsion also inhibited the NK activity of NK-enriched effector cells. Decreased cytotoxicity was first noted 24 hr after injection; it resumed the baseline by 7 days. The addition of EPA-TG emulsion to a cytotoxicity assay system resulted in moderate depression of NK activity. These results demonstrate that EPA has significant immunomodulatory effects on NK activity.},
doi = {10.1016/0006-291X(88)90548-7},
url = {https://www.osti.gov/biblio/5471684}, journal = {Biochem. Biophys. Res. Commun.; (United States)},
number = ,
volume = 150:1,
place = {United States},
year = {Fri Jan 15 00:00:00 EST 1988},
month = {Fri Jan 15 00:00:00 EST 1988}
}