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Title: Separate photosensitizers mediate degradation of the 32-kDa photosystem II reaction center protein in the visible and UV spectral regions

Abstract

A component of the photosystem II reaction center, the 32-kDa protein, is rapidly turned over in the light. The mechanism of its light-dependent metabolism is largely unknown. We quantified the rate of 32-kDa protein degradation over a broad spectral range (UV, visible, and far red). The quantum yield for degradation was highest in the UVB (280-320 nm) region. Spectral evidence demonstrates two distinctly different photosensitizers for 32-kDa protein degradation. The data implicate the bulk photosynthetic pigments (primarily chlorophyll) in the visible and far red regions, and plastoquinone (in one or more of its redox states) in the UV region. A significant portion of 32-kDa protein degradation in sunlight is attributed to UVB irradiance.

Authors:
; ; ; ; ;  [1]
  1. Weizmann Institute of Science, Rehovot (Israel)
Publication Date:
OSTI Identifier:
5467823
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 86:17; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHLOROPHYLL; RADIOSENSITIVITY EFFECTS; PHOTOSYNTHETIC REACTION CENTERS; CHEMICAL RADIATION EFFECTS; PROTEINS; METABOLISM; QUINONES; DOSE-RESPONSE RELATIONSHIPS; MOLECULAR WEIGHT; PHOTOSYNTHESIS; PLANTS; ULTRAVIOLET RADIATION; AROMATICS; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; CHEMISTRY; ELECTROMAGNETIC RADIATION; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PHYTOCHROMES; PIGMENTS; PORPHYRINS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; SYNTHESIS; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Greenberg, B M, Gaba, V, Canaani, O, Malkin, S, Mattoo, A K, and Edelman, M. Separate photosensitizers mediate degradation of the 32-kDa photosystem II reaction center protein in the visible and UV spectral regions. United States: N. p., 1989. Web. doi:10.1073/pnas.86.17.6617.
Greenberg, B M, Gaba, V, Canaani, O, Malkin, S, Mattoo, A K, & Edelman, M. Separate photosensitizers mediate degradation of the 32-kDa photosystem II reaction center protein in the visible and UV spectral regions. United States. https://doi.org/10.1073/pnas.86.17.6617
Greenberg, B M, Gaba, V, Canaani, O, Malkin, S, Mattoo, A K, and Edelman, M. 1989. "Separate photosensitizers mediate degradation of the 32-kDa photosystem II reaction center protein in the visible and UV spectral regions". United States. https://doi.org/10.1073/pnas.86.17.6617.
@article{osti_5467823,
title = {Separate photosensitizers mediate degradation of the 32-kDa photosystem II reaction center protein in the visible and UV spectral regions},
author = {Greenberg, B M and Gaba, V and Canaani, O and Malkin, S and Mattoo, A K and Edelman, M},
abstractNote = {A component of the photosystem II reaction center, the 32-kDa protein, is rapidly turned over in the light. The mechanism of its light-dependent metabolism is largely unknown. We quantified the rate of 32-kDa protein degradation over a broad spectral range (UV, visible, and far red). The quantum yield for degradation was highest in the UVB (280-320 nm) region. Spectral evidence demonstrates two distinctly different photosensitizers for 32-kDa protein degradation. The data implicate the bulk photosynthetic pigments (primarily chlorophyll) in the visible and far red regions, and plastoquinone (in one or more of its redox states) in the UV region. A significant portion of 32-kDa protein degradation in sunlight is attributed to UVB irradiance.},
doi = {10.1073/pnas.86.17.6617},
url = {https://www.osti.gov/biblio/5467823}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 86:17,
place = {United States},
year = {Fri Sep 01 00:00:00 EDT 1989},
month = {Fri Sep 01 00:00:00 EDT 1989}
}