Phosphoinositide metabolism and adrenergic receptors in astrocytes
Agonist-induced phosphoinositide (PI) breakdown functions as a signal generating system. Diacylglycerol, one breakdown product of phosphotidylinositol-4,5-diphosphate hydrolysis, can stimulate protein kinase C, whereas inositol triphosphate, the other product, has been proposed to be a second messenger for Ca/sup + +/ mobilization. Using purified astrocyte cultures from neonatal rat brain, the effects of adrenergic agonists and antagonists at 10/sup -5/ M were measured on PI breakdown. Astrocytes grown in culture were prelabeled with (/sup 3/H)inositol, and basal (/sup 3/H) inositol phosphate (IP/sub 1/) accumulation was measured in the presence of Li/sup +/. Epinephrine > norepinephrine (NE) were the most active stimulants of IP/sub 1/ production. The ..cap alpha../sub 1/ adrenoreceptor blockers, phentolamine and phenoxybenzamine, added alone had no effect on IP/sub 1/ production was reduced below basal levels. Propranolol partially blocked the effects of NE. Clonidine and isoproterenol, separately added, reduced IP/sub 1/ below basal levels and when added together diminished IP/sub 1/ accumulation even further. The role of adrenergic stimulation in the production of c-AMP.
- Research Organization:
- Univ. of California, Los Angeles
- OSTI ID:
- 5450358
- Report Number(s):
- CONF-8604222-; TRN: 86-028411
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Inositol phospholipid hydrolysis in cultured astrocytes and oligodendrocytes
Regulation of Na-Cl absorption in rabbit proximal colon in vitro
Related Subjects
INOSITOL
METABOLISM
SYMPATHOMIMETICS
RECEPTORS
ADRENALINE
ANIMAL CELLS
BRAIN
CALCIUM
CATIONS
CELL CULTURES
LITHIUM COMPOUNDS
NEONATES
NERVE CELLS
NORADRENALINE
PHOSPHATES
PHOSPHOTRANSFERASES
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CHARGED PARTICLES
DRUGS
ELEMENTS
ENZYMES
HORMONES
INOSITOLS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
METALS
MONOSACCHARIDES
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RODENTS
SACCHARIDES
SOMATIC CELLS
STEROID HORMONES
TRANSFERASES
VERTEBRATES
550501* - Metabolism- Tracer Techniques