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Title: Imaging neurotransmitter uptake and depletion in astrocytes

Abstract

An ultraviolet (UV) laser-based optical microscope and charge-coupled device (CCD) detection system was used to obtain chemical images of biological cells. Subcellular structures can be easily seen in both optical and fluorescence images. Laser-induced native fluorescence detection provides high sensitivity and low limits of detection, and it does not require coupling to fluorescent dyes. We were able to quantitatively monitor serotonin that has been taken up into and released from individual astrocytes on the basis of its native fluorescence. Different regions of the cells took up different amounts of serotonin with a variety of uptake kinetics. Similarly, we observed different serotonin depletion dynamics in different astrocyte regions. There were also some astrocyte areas where no serotonin uptake or depletion was observed. Potential applications include the mapping of other biogenic species in cells as well as the ability to image their release from specific regions of cells in response to external stimuli. {copyright} {ital 1997} {ital Society for Applied Spectroscopy}

Authors:
 [1];  [2];  [1]
  1. Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011 (United States)
  2. Department of Zoology and Genetics, Laboratory of Cellular Signaling, Iowa State University, Ames, Iowa 50011 (United States)
Publication Date:
Research Org.:
Ames National Laboratory
OSTI Identifier:
544776
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Applied Spectroscopy
Additional Journal Information:
Journal Volume: 51; Journal Issue: 8; Other Information: PBD: Aug 1997
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; NERVE CELLS; IMAGE PROCESSING; SEROTONIN; OPTICAL MICROSCOPY; FLUORESCENCE; CHARGE-COUPLED DEVICES; ULTRAVIOLET SPECTRA; SPATIAL RESOLUTION

Citation Formats

Tan, W, Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, Haydon, P G, and Yeung, E S. Imaging neurotransmitter uptake and depletion in astrocytes. United States: N. p., 1997. Web. doi:10.1366/0003702971941656.
Tan, W, Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, Haydon, P G, & Yeung, E S. Imaging neurotransmitter uptake and depletion in astrocytes. United States. doi:10.1366/0003702971941656.
Tan, W, Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200, Haydon, P G, and Yeung, E S. Fri . "Imaging neurotransmitter uptake and depletion in astrocytes". United States. doi:10.1366/0003702971941656.
@article{osti_544776,
title = {Imaging neurotransmitter uptake and depletion in astrocytes},
author = {Tan, W and Department of Chemistry, University of Florida, Gainesville, Florida 32611-7200 and Haydon, P G and Yeung, E S},
abstractNote = {An ultraviolet (UV) laser-based optical microscope and charge-coupled device (CCD) detection system was used to obtain chemical images of biological cells. Subcellular structures can be easily seen in both optical and fluorescence images. Laser-induced native fluorescence detection provides high sensitivity and low limits of detection, and it does not require coupling to fluorescent dyes. We were able to quantitatively monitor serotonin that has been taken up into and released from individual astrocytes on the basis of its native fluorescence. Different regions of the cells took up different amounts of serotonin with a variety of uptake kinetics. Similarly, we observed different serotonin depletion dynamics in different astrocyte regions. There were also some astrocyte areas where no serotonin uptake or depletion was observed. Potential applications include the mapping of other biogenic species in cells as well as the ability to image their release from specific regions of cells in response to external stimuli. {copyright} {ital 1997} {ital Society for Applied Spectroscopy}},
doi = {10.1366/0003702971941656},
journal = {Applied Spectroscopy},
number = 8,
volume = 51,
place = {United States},
year = {1997},
month = {8}
}